Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Fixation and Sectioning01:03

Fixation and Sectioning

4.5K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.5K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.5K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Global survey of microscopy core facilities.

Journal of microscopy·2026
Same author

Global survey of Flow Cytometry core facilities 2021.

Journal of microscopy·2026
Same author

Contextual High-Throughput 3D Volume Electron Microscopy Data Acquisition Using Artificial Intelligence.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same author

NCR13 peptide protects soybean against Cercospora sojina using multi-faceted modes of action and additive interaction with chemical fungicides.

Journal of experimental botany·2025
Same author

Best practices in plant fluorescence imaging and reporting: A primer.

The Plant cell·2025
Same author

Plants in the expansion era: recent advances in expansion microscopy in plant systems.

Journal of experimental botany·2025

相关实验视频

Updated: Jul 23, 2025

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
11:48

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Published on: May 17, 2022

4.0K

一种用于植物的常规固定体积电子显微镜协议.

Janithri S Wickramanayake1, Kirk J Czymmek1

  • 1Donald Danforth Plant Science Center, Saint Louis, MO, United States; Advanced Bioimaging Laboratory, Donald Danforth Plant Science Center, Saint Louis, MO, United States.

Methods in cell biology
|July 14, 2023
PubMed
概括

这项研究介绍了植物组织的新化学固定协议,使细胞结构的高分辨率体积电子显微镜 (EM) 成为可能. 优化的方法克服了植物样本特性所带来的挑战,用于先进的电磁成像.

科学领域:

  • 植物生物学 植物生物学
  • 显微镜的使用方法
  • 细胞生物学 细胞生物学

背景情况:

  • 体积电子显微镜 (EM) 对植物研究至关重要,但植物组织对高分辨率成像具有独特的挑战.
  • 挑战包括大空隙,细胞壁和状皮质,阻碍形态,染色和导电性.

研究的目的:

  • 开发和验证一个强大的化学固定协议,用于为高分辨率体积EM准备植物样本.
  • 为解决与植物组织相关的特定挑战,采用诸如串行块面扫描电子显微镜 (SB-SEM) 和聚焦离子束扫描电子显微镜 (FIB-SEM) 等技术.

主要方法:

  • 采用了经过修改的化学固定策略,首先使用甲甲和甲,然后再使用四氧化物,铁化物,硫碳水化合物和四氧化物进行二次固定.
  • 进一步染色涉及乌兰酸和酸,在中脱水,并嵌入Quetol 651树脂.
  • 用SB-SEM与焦点电荷补偿成像样本.

主要成果:

  • 该协议成功地产生了烟草和叶叶组织中细胞结构的高对比图像.
  • 关键的器官,如线粒体,戈尔吉,内质网膜和核外膜被清晰地可视化.
  • 叶绿体甲状腺膜和花叶片明显分离,证明了该方案的有效性.

结论:

关键词:
хлоропласт 是一种质塑料.草是一种草.聚焦离子束扫描电子显微镜尼科蒂亚娜本塔米亚娜 (Nicotiana benthamiana) 是一种植物.这就是OTO OTO.奥斯-二碳水化合物-奥斯连续块面扫描电子显微镜.烟草 烟草 烟草 烟草 烟草

更多相关视频

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples
10:57

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

29.1K
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
12:52

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

16.5K

相关实验视频

Last Updated: Jul 23, 2025

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
11:48

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Published on: May 17, 2022

4.0K
Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples
10:57

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

29.1K
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
12:52

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

16.5K

  • 这种优化的样本准备协议为常规植物体积电子显微镜提供了可靠的方法.
  • 它有效地克服了先进的EM技术用于植物组织制备的常见挑战.
  • 该协议促进了植物细胞和组织的详细超结构分析.