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相关概念视频

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.3K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K
Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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相关实验视频

Updated: Jul 23, 2025

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

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积极的监管要素之间的超远程相互作用.

Elias T Friman1, Ilya M Flyamer2, Davide Marenduzzo3

  • 1MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh EH4 2XU, United Kingdom; Elias.Friman@ed.ac.uk Wendy.Bickmore@ed.ac.uk.

Genome research
|July 14, 2023
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概括
此摘要是机器生成的。

活跃的调节元素在巨大的基因组距离之间相互作用,由多价值结合因子和不同的过程驱动,如凝聚素介导的循环挤出,多接触和活跃区域聚类. 这澄清了基因调节机制.

更多相关视频

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 基因规则 基因规则

背景情况:

  • 增强剂和促进剂之间的染色体相互作用对于基因转录激活至关重要.
  • 了解驱动这些相互作用的因素是解读基因调节的关键.

研究的目的:

  • 为了研究促使调节元素之间的染色体相互作用的因素.
  • 分析接触频率和DNA结合因子与这些相互作用的关联,跨越大基因组距离.

主要方法:

  • 利用染色体构造捕获 (3C) 数据来确定数百万个 cis-调节元素之间的接触频率.
  • 在具有丰富接触的区域分析了数百个DNA结合因子的结合.
  • 采用模拟来建模染色体相互作用和多价值结合因子.

主要成果:

  • 鉴定了与活跃转录相关的因子结合的部位的丰富接触.
  • 证明了活跃的调节元素在不同基因组的几十个大基底之间相互作用,独立于凝聚素和多基因组.
  • 显示这些超远程相互作用并不依赖于RNA聚合酶II或单个转录辅因子.
  • 模拟支持了一个模型,其中多价值结合因子通过桥接诱导的集群驱动远程相互作用.

结论:

  • cis调节元件之间的远程相互作用是由多个不同的过程驱动的.
  • 这些过程包括凝聚素介导的循环挤出,多接触和活跃区域的集群.
  • 活跃的调节元素可以在超长的距离上相互作用,受到因子聚类的影响,并且独立于转录机制.