Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

72
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
72
CRISPR01:59

CRISPR

52.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
Homologous Recombination02:31

Homologous Recombination

50.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.7K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

60
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
60

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A diffusion-based 3D printing strategy to fabricate self-supporting, perfusable networks.

BMC methods·2026
Same author

Decoding skin aging: Emerging roles of miRNAs, lncRNAs, and circRNAs in mechanisms, therapies, and future horizons.

Ageing research reviews·2026
Same author

Swertisin improves Alzheimer's disease-like pathology in 5XFAD male mice through regulation in plasmin activity.

Neurochemistry international·2026
Same author

Single-Molecule DNA Tweezers Enable Programmable Control of Enzyme Activity via Arbitrary Molecular Cues.

Angewandte Chemie (International ed. in English)·2025
Same author

Identification of α-Azacyclic Acetamide-Based Inhibitors of <i>P. falciparum</i> Na<sup>+</sup> Pump (<i>Pf</i>ATP4) with Fast-Killing Asexual Blood-Stage Antimalarial Activity by Phenotypic Screening.

ACS infectious diseases·2025
Same author

33 Unresolved Questions in Nanoscience and Nanotechnology.

ACS nano·2025

相关实验视频

Updated: Jul 23, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.1K

通过纳米技术增强CRISPR/Cas系统

Rupali Chowdhry1, Steven Z Lu2, Seungheon Lee3

  • 1Department of Public Health, The University of Texas at Austin, Austin, TX 78712, USA.

Trends in biotechnology
|July 14, 2023
PubMed
概括

纳米技术增强了CRISPR/Cas系统,以改善细胞进入,稳定性和诊断和治疗中的向传递. 这种整合承诺更敏感的检测和个性化治疗,克服当前的局限性.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?诊断 诊断 诊断 诊断 诊断基因编辑 基因编辑纳米技术是纳米技术.治疗药物 治疗药物

更多相关视频

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.9K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.8K

相关实验视频

Last Updated: Jul 23, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.1K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.9K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.8K

科学领域:

  • 生物技术和纳米医学
  • 分子生物学和基因编辑

背景情况:

  • 克里斯普尔/卡斯系统在生物学和医学方面提供了革命性的潜力,使新的疾病诊断和治疗成为可能.
  • 目前的CRISPR/Cas系统面临的局限性包括细胞进入不良,生物环境的不稳定性和非目标效应.

研究的目的:

  • 探索纳米技术与CRISPR/Cas系统的整合,以克服现有的局限性.
  • 突出利用纳米材料提高CRISPR/Cas系统诊断和治疗性能方面的进展.

主要方法:

  • 关于CRISPR/Cas系统和纳米技术集成的最新科学文献的审查.
  • 分析纳米材料如何应对诸如细胞传递,稳定性和特异性等挑战.
  • 检查纳米技术增强的CRISPR/Cas用于诊断平台和治疗应用.

主要成果:

  • 纳米技术的整合增强了CRISPR/Cas系统的优势组织积累和可调节性质.
  • 纳米材料提高了CRISPR/Cas稳定性和细胞进入,减少了目标外影响.
  • 纳米材料可以实现更快,更灵敏,更方便的CRISPR/Cas介导检测平台.

结论:

  • 将纳米技术与CRISPR/Cas系统集成是克服当前局限性的有希望的策略.
  • 需要进一步开发才能充分实现纳米技术增强的CRISPR/Cas技术的潜力.
  • 这种跨学科的方法对诊断和治疗的进步具有重大前景.