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在清醒的小鼠中,对臂核活动的知觉和疼痛

Jesse A Smith¹, Yadong Ji², Rebecca Lorsung¹

¹Program in Neuroscience, Department of Neurobiology, School of Medicine, University of Maryland, Baltimore, Maryland 21201.

The Journal of neuroscience : the official journal of the Society for Neuroscience

|July 14, 2023

概括

研究人员记录了醒着的小鼠经历疼痛的副核 (PBN) 的活动. 在疼痛状态中,PNN神经元活动被放大,与兴奋相关,为疼痛感知提供了新的见解.

科学领域:

神经科学是一个神经科学.
疼痛研究疼痛研究
行为科学行为科学

背景情况:

双臂核复合体 (PBN) 对于处理厌恶和疼痛至关重要.
以前的研究表明,在麻醉动物的慢性疼痛期间,PBN神经元活动放大.

研究的目的:

开发一种方法来记录PBN神经元活动在有害刺激期间表现的小鼠.
为了研究不同疼痛模型的清醒动物中的PNN神经元活动.
探索PBN活动,疼痛和兴奋之间的关系.

主要方法:

开发了一种技术,可以从头部受限,表现的小鼠的PBN神经元中记录.
应用可重现的有害刺激,并使用光纤测量测量反应.
使用了表达PNN神经元的素基因相关 (CGRP).
在雄性和雌性小鼠中评估了神经病变和炎症性疼痛模型.
与行为疼痛指标和瞳孔区域 (兴奋) 相关联的PNN活动.

主要成果:

自发和唤起的PNN神经元活动在清醒的小鼠中明显高于麻醉的小鼠.
在神经病和炎症性疼痛模型中,PBN神经元对有害刺激的反应增强,持续至少5周.
在与有害刺激结合后,PBN神经元可以被调节以对无害刺激做出反应.
PBN神经元活动的变化与兴奋水平 (瞳孔区域) 的变化相关.

结论:

开发的方法允许对行为动物的PBN活动进行可靠的记录.
PBN神经元活动由疼痛状态和兴奋动态调节.
这些发现增强了我们对慢性疼痛感知和厌恶背后的神经回路的理解.

关键词:

这是CGRP的CGRP.麻醉是一种麻醉.厌恶厌恶厌恶慢性疼痛是一种慢性疼痛.有条件反应的条件响应.纤维光度仪的光度仪.

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