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潜在的候选基因和与Dianthus spiculifolius细胞质男性不育相关的途径,由转录组分析揭示

Yingzhu Liu¹, Han Sun¹, Rong Ye¹

¹College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.

Plant cell reports

|July 14, 2023

概括

在Dianthus spiculifolius中调查细胞质男性不育 (CMS),这项研究确定了差异表达的基因和途径. 引入DsHSP70到Arabidopsis诱导男性不育和花/花异常,提供了关于CMS机制的见解.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
生殖生物学生殖生物学

背景情况:

细胞质男性不育性 (CMS) 对于混合种子生产至关重要.
在Dianthus spiculifolius中CMS的调节机制尚不清楚.
在D. spiculifolius中,男性不育可能涉及面膜形和花粉发育受损.

研究的目的:

为了调查Dianthus spiculifolius.男性不育的遗传基础.
确定与CMS相关的差异表达基因 (DEG) 和调节途径.
评估候选基因DsHSP70在植物生殖发育中的功能.

主要方法:

对D. spiculifolius的雄性无菌和肥沃线的比较RNA测序.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
权重基因共同表达网络分析 (WGCNA).
在Arabidopsis thaliana中对DsHSP70的功能分析.

主要成果:

确定了12365个DEG,其中1765个在关键的其他发展阶段普遍表达.
DEGs主要参与氧化减氧,信号转导和编程细胞死亡.
WGCNA确定了三种可能与男性不育相关的模块.
在Arabidopsis中,DsHSP70的过度表达导致了另一次流产和异常的隔膜/花发育.

结论:

确定了D. spiculifolius中CMS的潜在候选基因和途径.
DsHSP70在雄性性质细胞的发育和花器官的完整性中起作用.
这项研究为Dianthus spiculifolius的CMS背后的分子机制提供了新的见解.

关键词:

这种植物是Dianthus spiculifolius.不同表达的基因.男人的不孕不育能力.编程细胞死亡编程细胞死亡转录组转录组就是一个转录组.

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