在面包小麦 (Triticum aestivum L.) 中,α-Tocopherol作为对抗UV-B压力的对策
Sedat Karaca1, Murat Aydin1, Güleray Agar2
1Department of Biotechnology, Faculty of Agriculture, Ataturk University, Erzurum, Turkey.
概括
紫外线 (UV-B) 辐射会损害小麦的生长,减少的发育和植物的再生. 外源性α-托科菲罗 (α-托科菲罗) 应用显著改善了这些参数,最高度为UV-B应激耐受性提供了最佳结果.
科学领域:
- 植物科学 植物科学
- 生物化学 生化学
- 环境压力生物学
背景情况:
- 光自营养有机体利用光能进行碳固定.
- 紫外线B (UV-B) 辐射,尽管臭氧层吸收,到达地球表面,对植物造成非生物压力.
- 由于植物的性,它们不断地受到环境压力,如UV-B辐射,这会阻碍生长并诱导细胞死亡.
研究的目的:
- 为了研究外源性α-托科菲罗尔 (α-托科菲罗尔) 对小麦中UV-B辐射诱导的压力的保护作用.
- 量化不同时间的紫外线B辐射对小麦的生长和再生的影响.
- 为了确定α-托科菲罗尔的最佳度,以提高小麦对UV-B应激的耐受性.
主要方法:
- 小麦胚胎被培养成卡利,并经过不同时间 (0,2,4,6小时) 的UV-B辐射.
- 随后,Calli在20天的时间内在含有不同度的α-托哥法醇 (0,50,100毫克/升) 的介质上进行培养.
- 通过将胚胎转移到再生介质中30天来评估再生潜力.
主要成果:
- 增加的紫外线B照射持续时间显著降低了的相对生长率,胚胎性的速度,响应胚胎性的速度和植物再生.
- 应用α-托科菲罗尔改善了UV-B应激对所有测量参数的负面影响.
- 在UV-B压力下增强植物再生的最有效度是100 mg/l的α-托哥菲罗尔.
结论:
- 外源性α-托科菲罗尔赋予小麦中的UV-B辐射应激显著的耐受性.
- 补充α-托科菲罗尔是一种有前途的策略,可以在UV-B压力条件下改善植物再生.
- 优化α-托科法醇的应用可以减轻UV-B辐射对植物发育的有害影响.
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