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梅罗特葡萄树中的奇米拉是由分阶段组装揭示的

V Sichel¹, G Sarah^1,2, N Girollet³

¹UMR AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, F-34398, France.

|July 14, 2023

概括

这项研究开发了一种新方法,使用先进的测序和生物信息学来检测葡萄藤中的危临床嵌合体. 这一发现有助于通过更好地了解遗传变异来改善葡萄酒品种.

科学领域:

植物遗传学植物遗传学
葡萄种植葡萄种植葡萄种植
基因组学就是基因组学.

背景情况:

在一个个体内存在多个基因型的化学性,对植物本体生殖和育种有价值,特别是在葡萄种植中对果颜色等特征有价值.
周临床嵌合体,其变异仅限于细胞层,是稳定的和可传播的,但关键的农业学特征可能会受到未识别的嵌合体的影响.
葡萄树叶的发育涉及L1和L2等级层,而横向根源仅来自L2,通过比较根和叶子DNA来检测嵌合体.

研究的目的:

开发和应用一种新的方法,用于检测"Merlot"葡萄品种中的危临床嵌合体.
为了利用高分辨率测序和生物信息学来准确识别和表征化梅拉.
通过克隆选择和育种,探索奇梅拉检测的潜力,以改善葡萄藤品种.

主要方法:

使用了新一代Hifi长读序列和与父序列 ("Magdeleine Noire des Charentes"和"Cabernet Franc") 的三重组合,以实现"Merlot"品种的单元型解析组合.
构建了33到47个连接的伪分子,使得高分辨率的单元型对比和注释转移从PN40024 VCost.v3.3.
应用了严格的变异选择,并使用分子倒置探头 (MIPseq) 验证了发现的结果,以确认检测到的模拟物.

主要成果:

分别在Merlot-haplotype-CF和Merlot-haplotype-MG上检测到51个和53个"Merlot"特异性临床嵌合体,其中一些位于编码区域.
成功完成了"Merlot"葡萄树的哈普洛型解析组合和伪分子构造.
使用MIPseq验证了69%的检测位置子集,其中25%是可疑的,6%是无效的,证明了该方法的有效性.

结论:

通过使用先进的测序和生物信息学,建立了一种可靠的方法来检测葡萄藤中的危临床嵌合体.
在"Merlot"品种中识别了众多的仿真品,突出了它们对农业特征的潜在影响.
通过精确的嵌合体识别和在育种计划中的利用,为改进葡萄酒品种开辟了新的途径.

关键词:

奇梅拉 Chimera 是一个奇梅拉.这就是Hifi序列.阶段性组装组装的阶段性组装.葡萄酒 (Vitis vinifera) 是一种葡萄酒.整个基因组的整个基因组.

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