通过融合提高深层致命突变原生体的再生率,以促进高效的L-lysine发酵
Nan Li1, Jie Lu2, Zirui Wang2
1College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
BMC biotechnology
|July 14, 2023
概括
这项研究通过结合紫外线突变和原始质细胞融合,优化了大肠杆菌QDW中的L-lysine产量,显著提高了L-lysine产量. 这种新的方法增强了微生物细胞工厂的工业应用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 代谢工程是代谢工程.
背景情况:
- 氨酸是动物料和专用饮食产品中至关重要的成分.
- 提高应变对于高效的L-lysine发酵至关重要.
- 大肠杆菌QDW是生产L-lysine的关键菌株.
研究的目的:
- 通过优化大肠杆菌QDW菌株来增强L-氨酸的产生.
- 提高高致死性原生体的再生率.
- 结合多重突变和同源细胞融合技术,以改善菌株.
主要方法:
- 使用特定的酶解时间 (4小时) 和溶酶度 (0.8毫克/毫升) 优化原生细胞分离.
- 在高致死性原生体中采用紫外线变异 (7分钟) 和热无活化 (30分钟在85°C).
- 采用同源细胞融合和流细胞测量来进行菌株分析.
主要成果:
- 达到最佳的原生质形成 (90%) 和再生 (30%) 率.
- 成功生成了四个稳定的结合菌株大肠杆菌.
- QDW-UH3菌株的L-氨酸产量增加了7.2%,关键合成酶的表达增强.
结论:
- 紫外线突变和原始质细胞融合的结合方法有效地改善了L-氨酸的产生.
- 这种方法为有针对性的微生物细胞工厂建设提供了一个新的策略.
- 优化的菌株显示了工业L-lysine发酵的巨大潜力.
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