在小鼠前腔毛囊中,BPA干扰了粒粉细胞的发育和卵细胞的半转化
Yaqiu Li1,2, Sitong Liu1,2, Fan Gao1
1Center for Reproductive Medicine, Jilin Medical University, Jilin 132013, P.R. China.
Experimental biology and medicine (Maywood, N.J.)
|July 15, 2023
概括
双A (BPA) 通过损害颗粒细胞功能和卵细胞半分裂,破坏女性生殖细胞的发育. 这种环境内分泌干扰剂减少了间隙结沟通,改变了环林B1的表达,影响了生殖健康.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 毒理学 毒理学 毒理学
背景情况:
- 双A (BPA) 是一种已知的环境内分泌干扰物,影响女性生殖细胞发育.
- 通过BPA干扰卵细胞和花质细胞发育的精确机制仍然不完全理解.
研究的目的:
- 为了研究BPA对小鼠前毛囊发育,花质细胞功能和卵细胞半转化in vitro的影响.
- 阐明BPA诱导的生殖毒性背后的分子机制.
主要方法:
- 在11天内用BPA (10微克/毫升) 治疗小鼠前腔毛囊的体外培养.
- 评估毛囊附着区域,累积细胞扩张,以及卵细胞半变异恢复率.
- 分析蛋白质表达 (P53,PCNA,ERα,环素B1),荷尔蒙水平 (雌激素,孕激素),Cx37表达和差距连接通信.
主要成果:
- BPA促进了颗粒细胞增殖 (PCNA) 和雌激素受体α (ERα) 表达,同时增加了雌激素和孕分泌.
- 乙减少了累积细胞的扩张,Cx37表达和颗粒细胞和卵细胞之间的间隙结沟通.
- 通过改变生殖囊泡分解 (GVBD) 卵细胞中的环林B1表达,BPA影响了卵细胞半转化,降低了成熟率而不诱导亡.
结论:
- BPA 干扰了颗粒状细胞的发育和卵细胞半球变异的进展.
- 关键机制包括缺口连接通信的减少和GVBD卵细胞中改变的环林B1表达.
- 这些发现凸显了BPA通过干扰关键的细胞通信和介质过程来损害女性生育的能力.
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