相关实验视频

Updated: Jul 23, 2025

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

微RNA发现研究的承诺和危险:努力实现质量而不是数量

Emma K McIlwraith¹, Wenyuan He¹, Denise D Belsham^1,2

¹Department of Physiology, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.

|July 15, 2023

概括

微RNA研究虽然很先进,但由于缺乏标准化,面临着可复制性问题. 本综述提供了指导方针,并强调了微RNA (miRNA) 研究中常见的陷,以提高研究质量.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物技术是生物技术.

背景情况:

自1993年以来,微RNA (miRNA) 研究已经显著扩大,有许多专利和临床试验.
尽管对miRNA生物发生和功能有着先进的知识,但应用研究仍在努力实现标准化和可重复性.
在miRNA注释,量化,调制,目标预测,验证和循环miRNA研究等领域仍然存在挑战.

结论:

实施标准化协议对于推进miRNA研究的可靠性至关重要.
解决发现的陷将提高未来miRNA研究的质量和一致性.
本综述旨在引导研究人员进行更可复制和更有影响力的miRNA调查.

关键词:

细胞生物学细胞生物学这些指导方针是指导方针.在下丘脑中,下丘脑微RNAs 是一个微型RNA.陷陷陷是一个陷.

更多相关视频

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...