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来自Solanum tuberdsm的多糖的制备和分析

Wenjian Yang¹, Gangliang Huang¹

¹Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, China.

Ultrasonics sonochemistry

|July 15, 2023

概括

这项研究优化了Solanum tuberdsm多糖体 (STP) 的提取. 使用特定的超声波和Sevage试剂条件,从STP中去除蛋白质的最佳方法实现了93.14%的去除率.

科学领域:

食品科学食品科学食品科学
生物化学生物化学
自然产品化学化学

背景情况:

来自Solanum tuberdsm (STP) 的多糖类对各种应用有兴趣.
有效的提取和净化对于利用STP至关重要.
蛋白质污染可能会阻碍提取的多糖的功能和应用.

研究的目的:

为了优化热水提取原始Solanum tuberdsm多糖化合物 (STP).
开发和完善一种从STP中去除蛋白质和其他杂质的方法.
使用Sevage方法和响应表面方法 (RSM) 建立蛋白质去除的最佳条件.

主要方法:

使用热水提取原始STP.
蛋白质,颜料,小分子和盐被使用Sevage试剂,藻石和蒸水透析去除.
使用响应表面方法 (RSM) 来优化蛋白质去除过程.
确定了最佳的Sevage方法条件:350W超声波功率,20分钟的超声波时间,双倍脱蛋白化,以及1:1 (v/v) 多糖化物溶液与Sevage试剂的比率.

主要成果:

优化的Sevage方法实现了高93.14%的蛋白质去除率.
成功确定了从STP中有效去除蛋白质的特定参数.
净化过程有效地去除了蛋白质,颜料,小分子和盐.

结论:

这项研究成功优化了Solanum tuberdsm多糖的提取和净化 (STP).
已建立的Sevage方法条件为高纯度STP隔离提供了一种高效的方法.
优化的协议显著提高了用于潜在应用的提取的STP质量.

关键词:

分析分析分析来自Solanum tuberdsm的多糖类超声波辅助的提取方法

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