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脑脊液中的miRNA-21,miRNA-155和miRNA-182表达与多发性硬化症患者脑脊液中的炎症因素之间的关系

Behrouz Shademan¹, Mana Zakeri², Samane Abbasi³

¹Department of Medical Biology, Faculty of Medicine, EGE University, Izmir, Turkey.

Clinical neurology and neurosurgery

|July 15, 2023

概括

在多发性硬化症 (MS) 患者的脑脊液 (CSF) 中,特定的微RNA (miRNA) 和炎症标志物升高. 这些分子显示出在MS中中枢神经系统炎症的诊断生物标志物的潜力.

科学领域:

神经免疫学神经免疫学
分子生物学分子生物学
生物标志物发现发现

背景情况:

微RNAs (miRNAs) 调节神经元细胞中的炎症基因表达.
炎症是多发性硬化症 (MS) 发病的关键组成部分.
了解miRNA参与MS炎症至关重要.

研究的目的:

研究MS患者脑脊液 (CSF) 中miRNA表达和炎症标志物之间的关系.
为了确定中枢神经系统炎症的潜在miRNA生物标志物在MS.

主要方法:

用RT-PCR量化脑脊流中的miRNA-21,miRNA-155和miRNA-182水平.
与酶相关的免疫吸收试验 (ELISA) 测量了炎症性细胞因子 (IL-1β,IL-6,TNF-α).
定量度测量评估了高灵敏度C反应蛋白 (hs-CRP) 的水平.

主要成果:

在MS患者和对照人群中,特定miRNA和炎症标志物的CSF水平显著更高.
通过ROC分析,miRNA-21,miRNA-182和miRNA-155证明了MS的高诊断准确性.
在CSF miRNA-155中,MS诊断的准确性最高.

结论:

脑流中IL-1β,IL-6,TNF-α,hs-CRP和特定的miRNA水平是MS中中枢神经系统炎症的有价值的生物标志物.
这些生物标志物可以帮助评估多发性硬化症中神经退行过程.
这项研究强调了miRNAs在MS相关炎症中的作用.

关键词:

炎症标志物是一种炎症标志物.微RNAs是一种微RNA.多发性硬化症 (MS) 是一种疾病.接收器的操作特征接收器的操作特征

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...