在芯片上的微流体生物传感器中增强检测内分泌干扰化学物质,使用aptamer介导的桥梁化和上转换发光
Jizhong Wu1, Wenya Wei1, Waqas Ahmad1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Journal of hazardous materials
|July 15, 2023
概括
这项研究引入了一种新型的微流体生物传感器,用于检测像双甲 (BPA) 和乙醇 (DES) 这样的内分泌干扰化学物质 (EDC). 创新的aptamer介导的纳米粒子桥接花增强了检测灵敏度,以改善人类健康监测.
科学领域:
- 纳米技术纳米技术
- 分析化学 分析化学
- 环境科学 环境科学
背景情况:
- 内分泌干扰化学物质 (EDC) 对人类健康构成重大风险.
- 精确和灵敏地检测EDC如双A (BPA) 和乙醇 (DES) 是至关重要的.
- 现有的检测方法可能缺乏灵敏度或需要复杂的程序.
研究的目的:
- 开发一种新的芯片上的微流体生物传感器,用于同时检测BPA和DES.
- 为了提高上转化纳米粒子 (UCNPs) 的发光和灵敏度,用于EDC检测.
- 为了研究在生物传感中以阿普他默为媒介的桥梁花机制的有效性.
主要方法:
- 用 (Tm) 和 (Er) 添加的核心外结构的UCNP的制造.
- 实施一种以阿普坦酶为媒介的桥梁积策略,以放大发光信号.
- 设计和模拟具有集成混合,反应和分离功能的微流体芯片.
- 使用开发的芯片内生物传感器同时检测BPA和DES.
主要成果:
- 核心外结构增强了UCNP发光率,高达2.28倍 (Tm) 和1.72倍 (Er).
- 通过Aptamer介导的桥梁花显著提高了生物传感器的发光率,增加了8.94倍 (Tm) 和7.10倍 (Er).
- 微流体生物传感器实现了BPA (0.0076微克L-1) 和DES (0.0131微克L-1) 的超敏感检测极限.
结论:
- 开发的芯片上的微流体生物传感器能够对BPA和DES进行高度敏感的同时检测.
- 通过aptamer介导的上转化纳米颗粒桥梁化是一种新且有效的策略,用于提高生物传感器性能.
- 这种方法为开发用于EDC监测的稳定和敏感的纳米生物传感器提供了一个有希望的新方向.
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