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Wnt/β-catenin信号控制着小鼠的眼生长,通过介导表皮-介质细胞相互作用

Xuming Zhu¹, Makoto Senoo², Sarah E Millar³

¹Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA; Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA.

The ocular surface

|July 15, 2023

概括

Wnt/β-catenin信号传递对于小鼠眼发育至关重要. 通过Wnt配体或β-catenin破坏这种途径,会阻止眼的生长,影响眼的增殖.

科学领域:

发育生物学是发展生物学.
分子信号通道的分子信号通道.

背景情况:

Wnt/β-catenin信号传递是一种调节细胞增殖,分化和组织模式的保存途径.
它在小鼠眼形态发生过程中的确切作用仍然不完全理解.

研究的目的:

阐明Wnt/β-catenin信号传递在小鼠眼发育中的功能.
为了研究表皮Wnt配体,介质细胞Wnt/β-catenin激活,以及眼形成中的p63之间的相互作用.

主要方法:

在特定胚胎组织中对Wnt/β-catenin通路组件 (β-catenin, Wls) 的遗传操纵.
条件淘汰p63以评估其监管作用.
分析眼形态,细胞增殖 (免疫光),细胞亡 (TUNEL试验) 和基因表达 (in situ杂交).

主要成果:

介质细胞β-catenin缺失取消了眼的生长,减少了相邻表皮和介质细胞中的增殖.
皮质Wls的删除导致了眼发育失败,反映了介质细胞β-catenin损失的影响.
p63淘汰导致眼低可塑性,并减少了表皮中的Wnt配体表达.

结论:

皮质Wnt配体对于激活介质细胞Wnt/β-catenin信号来说至关重要,这推动了眼的生长.
p63部分调节了这些关键的Wnt配体的表达.
这项研究突出了控制哺乳动物眼发育的关键信号轴.

关键词:

皮质-介质细胞相互作用眼的发育眼的发育在Fgf10中,Fgf10是什么意思?核武器的扩散信号通道的信号通道.这就是为什么Wls Wls.他们不想做.在p63中,p63是什么?这是一种β-catenin.

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