相关实验视频

Updated: Jul 23, 2025

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
09:14

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

11.6K

从Bacillus aryabhattai通过Bacillus subtilis的非经典蛋白质分泌途径有效分泌自然细胞内β-银酸酶的表达

Xuguo Duan1, Shuyue Luan1

  • 1College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.

International journal of biological macromolecules
|July 15, 2023
PubMed
概括

细菌细菌通过非经典的途径有效分泌细胞内β-银酸酶 (lacZBa),实现高产量和提高酶稳定性. 这种方法绕过了传统的信号,提高了细胞外酶的产生.

关键词:
非经典的蛋白质分泌途径再组合的表达方式.这就是β-Galactosidase.

更多相关视频

Transient Expression of Foreign Genes in Insect Cells sf9 for Protein Functional Assay11:12

Transient Expression of Foreign Genes in Insect Cells sf9 for Protein Functional Assay

Published on: February 22, 2018

12.5K
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

33.6K

相关概念视频

Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
39
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.6K
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
47