酵母的mRNA结合蛋白Cth2在转录后调节埃尔戈斯特醇生物合成,以应对缺铁
Tania Jordá1, Nicolas Rozès2, María Teresa Martínez-Pastor3
1Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain.
概括
缺铁会降低真菌埃尔戈斯特醇生物合成的调节. 铁调节蛋白Cth2限制了关键的欧戈斯特醇基因的翻译和mRNA水平,影响了膜完整性.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 固醇合成是真核生物中关键的,依赖于铁的代谢途径.
- 众所周知,在缺乏铁的条件下,真菌的ergosterol生物合成 (ERG) 被抑制.
研究的目的:
- 研究铁调节的mRNA结合蛋白Cth2在Saccharomyces cerevisiae中调节ergosterol生物合成中的作用.
- 阐明Cth2影响ERG基因表达的分子机制.
主要方法:
- 在铁限制和Cth2过度表达下分析ERG基因表达 (ERG1,ERG7,ERG11).
- 研究Cth2对mRNA水平的影响以及目标ERG基因的翻译.
- 评估固醇中间体积累 (二甲烯) 和固醇水平.
- 对酵母细胞对膜完整性压力的反应 (乙醇,醇) 的评估.
主要成果:
- 铁的限制或Cth2的过度表达降低了厄戈斯特醇合成 (ERG1,ERG7,ERG11) 的初始酶步骤.
- Cth2限制了翻译,并降低了含有AU丰富元素 (ARE) 的特定ERG基因的mRNA水平.
- CTH2的表达导致烯的积累,并降低了厄戈斯特醇水平.
- 改变CTH2表达影响酵母细胞对膜完整性的耐受性.
结论:
- 在酵母中缺铁期间,Cth2是ergosterol生物合成的关键调节因子.
- 通过控制ERG基因表达,Cth2调节铁依赖的固醇合成.
- 在压力条件下,Cth2在维持膜完整性方面发挥着重要作用.
相关概念视频
01:56Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
01:28Yeast Signaling
14.7K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.7K
01:23Stringent Response in E. coli
31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31
01:23Transcriptional Regulation: Riboswitches
61
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
61
01:29Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45
01:17Gene Regulation During Sporulation
36
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
36