通过加权基因共同表达网络分析,识别昆苗中与不同含量相关的核心基因
Shan Zhang1, Jian Liu2, Lian Shi3
1College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
BMC genomics
|July 15, 2023
概括
研究人员在子苗中发现了调节水平的关键基因,有助于培育具有改善利用率和抗非生物应激性作物的作物.
科学领域:
- 植物基因组学 植物基因组学
- 农业科学 农业科学
- 分子生物学分子生物学
背景情况:
- 昆是一种营养丰富的作物,具有显著的非生物应激抵抗力.
- 昆的生长受到其低效的土壤利用的限制.
- 有限的研究存在于昆苗的水平反应.
研究的目的:
- 为了识别参与昆对不同度的反应的核心基因.
- 为了分析不同处理下昆苗的转录组数据.
主要方法:
- 权重基因共同表达网络分析 (WGCNA) 应用于转录组数据.
- 分析了12,085个基因的基因表达数据.
- 进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
主要成果:
- 两个基因共同表达模块与水平有很强的相关性.
- 确定了五个核心基因,与水平有显著的正或负相关性.
- 揭示了参与反应的关键生物过程和代谢途径.
结论:
- 确定了关键的核心基因,包括转录因子 (MYB,WRKY,ERF).
- 这些基因对无生物应激抵抗和水平响应至关重要.
- 这种基因库将促进对耐受性昆品种的繁殖.
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