相关实验视频

Updated: Jul 23, 2025

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

DENND2B在细胞间桥上激活Rab35,调节细胞因子解离和四化

Rahul Kumar¹, Vincent Francis¹, Maria S Ioannou¹

¹Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC, Canada.

|July 16, 2023

概括

DENND2B作为Rab35的瓜核酸交换因子 (GEF),对细胞分裂 (细胞动力学) 起至关重要的作用. 它的功能障碍延迟了细胞分离,导致多核细胞并激活细胞循环检查点.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

细胞动力学,细胞分裂过程,涉及由Rab GTPases及其GEFs调节的膜贩运.
有效的细胞动力学需要脱离,这是一个最终的膜裂变阶段,取决于actin脱聚合.
已知Rab35和MICAL1在脱离过程中调节了actin动态.

研究的目的:

研究DENND2B在细胞动力学和切割中的作用.
为了确定DENND2B是否作为Rab GTPases的关氨酸核酸交换因子 (GEF).
阐明DENND2B调节actin动态和脱离的分子机制.

主要方法:

细胞培养和淘汰实验 (siRNA) 来消耗DENND2B.
免疫光显微镜可用于可视化F-actin,ESCRT-III和Aurora B激酶.
同免疫沉试验用于研究蛋白质相互作用.
分析多核和染色体桥梁作为脱离失败的指标.

主要成果:

DENND2B 作为 Rab35 GEF 的功能,在细胞间细胞动力桥 (ICB) 上招募和激活 Rab35.
DENND2B与活跃的Rab35相互作用,表明GEF和效应者的双重作用.
DENND2B的耗尽导致延迟脱离,F-actin积累,ESCRT-III招募受损,以及NoCut/脱离检查点的激活.
DENND2B的淘汰导致Aurora B激酶的激活,这是一个检查点参与的标记.

结论:

DENND2B 通过控制 Rab35 活性和行为动态,是细胞动力学除的关键调节者.
DENND2B的失调有助于细胞分裂错误,可能与癌症和先天性疾病有关.
DENND2B作为一个关键的GEF和Rab35的效应因子,在细胞脱离过程中整合了膜贩运和细胞骨调节.

关键词:

CP: 细胞生物学细胞生物学 DENN 域名 DENN 域名环保环境基金 (GEF) 已经开始了.拉布GTPases是一种GTPase.切割检查站的检查站.这就是Actin Actin.细胞动力学细胞动力学效应器效应器是一个有效的效应器.

更多相关视频

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

相关概念视频

The Contractile Ring

The Contractile Ring

Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...

Rab Cascades

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.

Cell Motility through Blebbing

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

Rab Proteins

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...