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Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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相关实验视频

Updated: Jul 23, 2025

Curtain Flow Column: Optimization of Efficiency and Sensitivity
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通过基于模型的优化对多列色谱配置进行比较.

Aditya Pareek1, Venkata Sudheendra Buddhiraju1, Vishnu Swaroopji Masampally1

  • 1TCS Research, Tata Research Development and Design Centre, Tata Consultancy Services, Pune, India.

Biotechnology progress
|July 16, 2023
PubMed
概括

基于模型的优化表明,三列周期逆流色谱 (PCC) 为单克隆抗体 (mAb) 净化提供了更高的产量和生产率. 然而,四列PCC在洗量限制下提供一致的性能.

关键词:
持续的生物处理.全球优化全球优化基于模型的优化优化这是一种单克隆抗体.多列色谱是多列色谱的一种方法.

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科学领域:

  • 生物制药制造业 生物制药制造业
  • 化学工程是化学工程的重要组成部分.
  • 过程优化 过程优化

背景情况:

  • 综合连续生物处理是生物制药制造业的未来.
  • 多列色谱提高了生物分子捕获的生产力和容量利用率.
  • 周期逆流色谱 (PCC) 是连续处理的一个关键技术.

研究的目的:

  • 优化两种流行的多列PCC设计 (3列和4列).
  • 根据单克隆抗体 (mAb) 料度和操作协议来评估性能.
  • 为了确定产量,生产力和容量利用的最佳配置.

主要方法:

  • 基于模型的优化使用辐射基础函数技术.
  • 对3列和4列PCC配置的分析.
  • 不同的mAb料度,现场清洗 (CIP) 和平衡持续时间.
  • 决定变量包括表面速度和阶段持续时间.

主要成果:

  • 在没有洗量限制的情况下,3列PCC在生产率,产量和容量利用率方面超过了4列PCC.
  • 在1.2 mg/mL mAb 料下,三列PCC实现了0.024 mg/mL的生产率,0.94的产量和0.94的容量利用率.
  • 在洗量限制的情况下,四列PCC显示出一致的生产率和产量,尽管容量利用率较低.

结论:

  • 当洗量不限时,在mAb净化中最大限度地提高产量和生产率时,3列PCC是优越的.
  • 四列PCC在更严格的操作约束下提供了稳定性和一致的性能,使其适合特定的制造场景.