自组装,体外基因传染,以及可生物降解的阳离子博拉的抗菌活性
Pabitra Mondal1, Joykrishna Dey1, Sadhana Roy2
1Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Langmuir : the ACS journal of surfaces and colloids
|July 16, 2023
概括
生物降解的阴离子球形被合成并研究了基因传递. 具有基基团的新型 bolaform 显示了与标准相比较的基因转染效率,在较高度下显著的细胞毒性和抗菌活性.
科学领域:
- 超分子化学 超分子化学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 博拉 (bolaforms) 越来越多地被研究用于药物和基因传递应用.
- 它们独特的结构使它们能够在水溶液中进行特定的自我组装.
- 开发具有增强基因转移的可生物降解的阴离子球形是一种关键的研究目标.
研究的目的:
- 设计和合成一种具有基头组的新型可生物降解的阴离子球形 (Bola-1).
- 调查Bola-1的自我组装行为和基因转染效率.
- 将Bola-1与其前体盐 (Bola-2) 进行比较,以了解基功能的作用.
主要方法:
- 新型阴离子球形 (Bola-1和Bola-2) 的合成.
- 使用表面张力,电导率,光探针,热量计,动态光散射和原子力显微镜进行物理化学表征.
- 在体外基因转移研究中,使用Hek 293细胞和小牛胸腺DNA.
主要成果:
- 这两种球形都形成了低临界聚合度 (CAC) 以上的球形或长长的.
- 基组显著影响了CAC值.
- 热量计数据表明,在没有盐的水中,热力学上有利的聚合物形成.
- 博拉形体表现出强大的DNA结合和基因转染效率,与球菌胺相美.
- 在高度下观察到显著的体外细胞毒性和抗菌活性.
结论:
- 成功合成和特征化了具有基功能的新型生物降解性阴离子球形.
- 这些球形细胞自组装成菌体,并表现出有前途的基因转染能力.
- 基组影响自我组装特性,并且这些化合物显示出治疗应用的潜力,以及观察到的细胞毒性和抗菌作用.
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