基于微型拉曼光谱学和数字微流体学的封闭式,一站式智能和精确的粒子表征
Han Sheng1, Liwen Chen2, Yinping Zhao1
1Institute of Biomedical Engineering and Technology, Academy for Engineer and Technology, Fudan University, 220 Handan Road, Shanghai, 200433, China.
Talanta
|July 16, 2023
概括
这项研究引入了一种新方法,使用数字微流体芯片上的微拉曼光谱来描述药物产品中的蛋白质颗粒. 该技术准确识别了八种颗粒类型,有助于质量控制和延长产品的保质期.
科学领域:
- 生物制药学分析分析
- 分析化学是一种分析化学.
- 材料科学是一种材料科学.
背景情况:
- 单克隆抗体可以由于制造和储存期间的压力而形成蛋白质颗粒.
- 控制可见微粒对于药物产品的质量和安全至关重要.
- 需要精确的粒子表征来理解应力条件并减轻风险.
研究的目的:
- 开发一种智能方法,在数字微流体芯片 (DMF) 上使用微拉曼光谱来表征蛋白质颗粒.
- 为了能够准确识别蛋白质颗粒类型及其压力来源.
- 改进控制蛋白质颗粒形成和延长药品保质期的策略.
主要方法:
- 使用应力降解生成蛋白质颗粒溶液.
- 在DMF芯片上的滴滴上进行了微拉曼光谱.
- 机器学习模型被训练在拉曼光谱上进行分类.
主要成果:
- 八种类型的蛋白质颗粒成功地以高准确度 (93%-100%) 进行了表征.
- 该方法提供了一个封闭的系统,小的样本体积,和组合形态/化学分析.
- 实现了简化数据分析和高分类准确度.
结论:
- 基于DMF的新型微拉曼光谱法使智能蛋白质颗粒的表征成为可能.
- 这种方法可以识别颗粒来源和应激条件,促进有针对性的控制策略.
- 该方法具有显著的潜力,可以提高生物制药产品的质量和保质期.
相关概念视频
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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