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外基因miR-21通过瘤微环境中的DGKB/ERK轴确定肺到脑转移的特异性

Tung-Yu Tiong¹, Mei-Lin Chan², Chun-Hua Wang³

¹Division of Thoracic Surgery, Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan; Division of Thoracic Surgery, Department of Surgery, Taipei Medical University-Shuang Ho Hospital, New Taipei City 235, Taiwan.

|July 16, 2023

概括

细胞外囊中的高miR-21促进肺癌大脑转移. 针对miR-21和ERK/STAT3通路使用ulixertinib显示了治疗承诺,可以减少转移并改善生存率.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症转移研究癌症转移研究

背景情况:

大脑转移是20-40%的肺癌患者的严重并发症.
在这些患者中,微RNA-21 (miR-21) 过度表达,并与ERK/STAT3通路有关.
这种途径的失调是潜在的治疗目标.

研究的目的:

研究miR-21在肺癌脑转移中的作用.
评估针对miR-21/ERK/STAT3途径对治疗结果的影响.
在细胞外囊泡 (EVs) 中分析miR-21及其转移性影响.

主要方法:

从转移性肺癌细胞中建立了瘤球体.
评估了miR-21水平,巨细胞两极分化和上皮细胞-介质细胞过渡.
分析了EV中的miR-21含量及其对ERK/STAT3信号和转移的影响.

主要成果:

瘤球体表现出高的miR-21,促进转移和上皮-介质细胞过渡.
来自球体的miR-21丰富的EV加速了ERK/STAT3信号和转移.
乌利克塞提尼布 (ERK抑制剂) 减少了转移,改善了小鼠的存活率,并减少了miR-21/EVs.

结论:

富含miR-21的EV显著加剧了肺到大脑转移.
用乌力谢丁尼布准miR-21/ERK/STAT3通路是一个有前途的策略.
这种方法为肺癌患者的脑转移管理提供了潜力.

关键词:

大脑转移的转移.癌症干细胞的利基是癌症干细胞.在 DGKB/ERK/STAT3 发送信号时.这是一种ERK抑制剂.细胞外囊泡 (EVs) 是一种细胞外囊泡.肺癌是一种肺癌.在OncomiR miR-21-21中使用.

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