来自Lagopsis supina的拉巴丹二烯酸及其抗神经炎症活性
Kai Wang1, Jiao-Tai Shi2, Ying Zhang3
1CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100039, China.
研究人员从Lagopsis supina中分离出了十种拉布丹二类植物,其中三种是新鲜的. 化合物4通过抑制微质细胞中氧化的释放,显示出显著的抗神经炎症活性潜力.
科学领域:
- 自然产品化学 自然产品化学
- 药理学 药理学是指药理学的学科.
- 神经科学是一个神经科学.
背景情况:
- Lagopsis supina 是一种生物活性化合物的植物来源.
- 拉巴丹二烯酸是一种具有多种生物活动的自然产品类.
- 神经炎症与各种神经系统疾病有关.
研究的目的:
- 从Lagopsis supina中分离和表征拉巴丹二类植物.
- 评估分离化合物的抗神经炎症潜力.
- 识别具有治疗应用的新型化合物.
主要方法:
- 从Lagopsis supina全植物中提取甲醇.整个植物.
- 使用HR-ESI-MS,1D和2DNMR进行结构阐明.
- 通过单晶X射线衍射来确定绝对配置.
- 在BV-2微质细胞上进行细胞活力测定 (CCK-8).
- 抑制脂多糖刺激的氧化 (NO) 生产的测定.
主要成果:
- 隔离了10种拉布丹二基 (1-10),其中包括3种新化合物 (1-3).
- 证实了五种化合物 (2-5,10) 的绝对配置.
- 所有分离的化合物对BV-2微质细胞没有显著的细胞毒性.
- 化合物4在10μM时显著抑制了脂聚糖诱导的NO释放.
结论:
- Lagopsis supina 是一个宝贵的labdane diterpenoids 的来源.
- 化合物4显示有前途的抗神经炎症特性.
- 对化合物4的作用机制进行进一步的研究对于潜在的治疗开发是有必要的.
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