使用环境线粒体DNA元编码对地表水中的多种便污染源的评估
Rose Ragot1, Florence Lessard2, André Bélanger2
1INRS Centre Armand-Frappier Santé Biotechnologie, 531 Boulevard des Prairies, Laval, QC, Canada, H7V 1B7.
The Science of the total environment
|July 16, 2023
概括
水源性疾病源于便污染. 线粒体DNA (mtDNA) 的元编码揭示了包括野生动物在内的多种动物来源,导致北克流域的污染并构成人类健康风险.
科学领域:
- 环境微生物学环境微生物学
- 分子生态学分子生态学
- 评估水质水质的评估.
背景情况:
- 水中传播的疾病是由便污染引起的,而同热物种是主要的水库.
- 便指标细菌 (FIB) 检测污染,但不检测污染源.
- 识别多个便来源 (人类,牲畜,野生动物) 需要特定的跟踪标记.
研究的目的:
- 为了扩大线粒体DNA (mtDNA) 的metabarcoding方法来评估表面水中的多个同热物.
- 在加拿大北克省L'Assomption河和Ville-Marie流域调查便污染源.
主要方法:
- 从北克的流域收集了86个地表水样本.
- 利用mtDNA元编码来识别存在的同热物种.
- 应用多变量分析和贝叶斯定理,将同热系与微生物和物理化学参数相关联.
主要成果:
- 在73%的样本中,便中的大肠杆菌水平超过了娱乐用水指南.
- 在40-88%的样本中检测到来自各种恒温动物 (人,牲畜,家禽,野生哺乳动物,野生鸟类) 的mtDNA.
- 在同热系 taxa 和便指标,人类人口和水质参数之间发现了显著的共变.
结论:
- 表面水中的便污染源于多种物种,包括野生动物.
- mtDNA元编码对于监测有助于便污染的各种动物物种至关重要.
- 多种便来源的存在凸显了对人类健康的潜在风险.
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