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在胰腺癌中miR-425-5p的表达及其与瘤免疫微环境的相关性

Shuo Jian¹, Dehua Kong¹, Jieli Tian¹

¹Department of Oncology, Suining Central Hospital, Suining, Sichuan Province, China.

Journal of investigative surgery : the official journal of the Academy of Surgical Research

|July 16, 2023

概括

微RNA-425-5p在胰腺癌 (PC) 中升高,并与抑制的免疫微环境 (TIME) 相关. 高的miR-425-5p表达表明PC患者的预后不佳.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

胰腺癌 (PC) 是一个重大的全球健康挑战,死亡率高.
微RNAs (miRNAs) 在瘤的开始和进展中起着至关重要的作用.
这项研究研究了miR-425-5p在PC中的表达及其与瘤免疫微环境 (TIME) 的关联.

研究的目的:

与相邻的非瘤组织相比,检查胰腺癌组织中miR-425-5p的表达水平.
确定miR-425-5p表达与瘤免疫微环境 (TIME) 之间的相关性,包括免疫细胞透和细胞因子概况.
在胰腺癌患者中评估miR-425-5p的预后价值.

主要方法:

使用定量实时PCR (RT-qPCR) 来测量miR-425-5p的表达.
使用流细胞计和ELISA量化免疫细胞群和细胞因子水平.
使用斯皮尔曼相关性分析,卡普兰-梅尔曲线和36个月的随访数据来评估与TNM阶段,时间和患者预后的关联.

主要成果:

发现miR-425-5p在PC组织上升调节,与晚期TNM阶段正相关.
PC组织表现出细胞毒性免疫细胞 (CD3 +,CD4 +,CD8 +,NK细胞) 和促炎性细胞因子 (IL-2,INF-γ) 的水平降低,以及调节性T细胞 (Tregs) 和免疫抑制性细胞因子 (IL-4,IL-10,TGF-β) 的增加.
miR-425-5p表达与Tregs,IL-10和TGF-β正相关,与细胞毒性免疫细胞和IL-2/INF-γ负相关,预测预后不佳.

结论:

在胰腺癌患者中,miR-425-5p被显著上调.
在PC中,miR-425-5p的表达与瘤免疫微环境 (TIME) 密切相关.
升高的miR-425-5p水平是胰腺癌预后不佳的预测标记.

关键词:

胰腺癌:胰腺癌是一种胰腺癌.免疫细胞免疫细胞免疫因素免疫因素在 miR-425-5p 中使用.预后预后预后

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