移动阶段和柱化学选择,用于高灵敏度非向的LC/ESI/HRMS水查
Amina Souihi1, Miklos Peter Mohai1, Jonathan W Martin2
1Department of Environmental and Materials Chemistry, Stockholm University, Svante Arrhenius väg 16, 106 91, Stockholm, Sweden.
Analytica chimica acta
|July 16, 2023
概括
对于非向的液体色谱-电子喷雾高分辨率质谱 (LC/ESI/HRMS),酸性移动相,如0.1%的酸,显著提高了分析物的灵敏度和特征检测. 这种优化对于分析复杂的样本至关重要,例如废水处理厂的流入和废水.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 优化移动相和柱化学对于敏感的液体染色学-电喷射高分辨率质谱学 (LC/ESI/HRMS) 来说至关重要.
- 非定向查存在挑战,原因是缺乏用于方法开发的预选化合物.
研究的目的:
- 为了比较各种移动阶段和列的性能,进行非目标LC/ESI/HRMS分析.
- 为选择最佳移动相提供一般指导,以最大限度地提高分析器的灵敏度和特征检测.
主要方法:
- 评估了20个移动相组合物和78个不同化合物的4个列.
- 通过不同方法比较分析物的灵敏度,重点关注pH值,添加剂,列类型和有机修饰剂.
- 在现实废水样本中评估了0.1%的酸,0.1%的氨和5.0mM的化,使用数据依赖的MS2获取和两个处理工作流 (MS-DIAL,patRoon).
主要成果:
- 酸性移动相,特别是0.1%的酸,显示了最高的分析剂灵敏度和反应因子.
- 移动相 pH,添加剂,柱化学和有机修饰剂含量显著影响了分析物的反应.
- 0.1%的酸在废水样本的全扫描 (MS1) 和碎片化谱 (MS2) 中产生了最多的检测特征.
结论:
- 0.1%的酸是非向LC/ESI/HRMS的高效移动相,提高了灵敏度和特征识别.
- 系统的移动阶段选择对于复杂的环境样本中强大的非目标选至关重要.
- 这些发现为LC/ESI/HRMS应用程序的方法开发提供了实际指导.
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