一种具有成本效益的微流体装置,用于教导电泳和电宇宙学原理
Tyler A Shaffer1, Carlos U Herrada2, Avery M Walker1
1C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, West Virginia 26505, United States.
Journal of chemical education
|July 17, 2023
概括
本研究介绍了一项低成本的实验室活动,用于教导电泳和电流. 学生可视化染料分离,展示极性和流量条件如何影响分析物迁移.
科学领域:
- 分析化学 分析化学
- 生物化学教育教育 生物化学教育
背景情况:
- 电泳原理在分析和生物化学中至关重要,但通常在先进的实验室中被教授.
- 实践经验对于理解电泳流动性和电流动性至关重要.
研究的目的:
- 呈现一个可访问的实验室活动,用于教学电泳和电流概念.
- 为了证明不同极度和流量条件如何影响迁移模式,分析迁移模式.
主要方法:
- 使用可重复使用的低成本仪器 (mini-E) 用常见试剂进行染料分离.
- 在酸性 () 和性 (氧化) 溶液中分离的闪亮蓝色FCF和紫红色AC食品染料.
- 在正常和逆极性条件下研究电泳运动和电流.
主要成果:
- 蓝色和红色染料的混合物成功地分成不同的带.
- 染料的迁移顺序在 (抑制电流) 和氧化 (活性电流) 条件之间有显著的变化.
- 学生表现出基于电和电泳原理预测分析物迁移的能力有所提高.
结论:
- 开发的实验室活动有效地教授了基本的电泳和电流的概念.
- 低成本和适应性使这项活动适合各种本科化学实验室体验.
- 了解电流是预测和解释电分离的关键.
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