设计甲基转化酵母Ogataea polymorpha以从甲醇中生产乳酸盐
Katrin Wefelmeier1, Simone Schmitz1, Anna Maria Haut1
1IAMB - Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, Aachen, Germany.
Frontiers in bioengineering and biotechnology
|July 17, 2023
概括
这项研究表明,甲基营养酵母Ogataea polymorpha可以从可持续的碳来源甲醇中产生乳酸盐. 改造的菌株实现了增长的改善,并保持了乳酸盐生产,为二氧化碳衍生化学制造铺平了道路.
科学领域:
- 生物技术和工业微生物学
- 合成生物学 合成生物学
- 可持续化学 可持续化学
背景情况:
- 乳酸是生物塑料的关键平台化学物质,如聚乳酸 (PLA).
- 目前的生产依赖于糖,促使对可持续的非食品原料进行研究.
- 从二氧化碳中提取的甲醇是一个有希望的替代碳来源.
研究的目的:
- 为了从甲醇中设计甲基转化酵母 Ogataea polymorpha 来生产乳酸盐.
- 为了优化发酵条件并增强甲醇同化,以改善乳酸位.
- 为在工业生物技术中利用二氧化碳衍生基质建立一个原则证明.
主要方法:
- 在甲醇可诱导促进剂下,在Ogataea polymorpha中表达四种不同的乳酸脱酶.
- 用不同的源和pH控制优化料批发发酵.
- 适应性实验室进化以增强工程菌株中的甲醇同化.
主要成果:
- 在Ogataea polymorpha.中成功表达和发挥Lactobacillus helveticusL-乳酸脱酶的功能.
- 改造品种在甲醇上表现出55%的增长率,同时保持乳酸生产.
- 使用进化菌株在用尿素养的料批培养中达到3.8g/L的最大乳酸标位.
结论:
- Ogataea polymorpha 是一种可行的宿主,可以从甲醇中生产乳酸盐.
- 该研究为基于二氧化碳的化学制造工程甲基型生物提供了基础.
- 这种方法支持可持续的工业生物技术,减少土地使用.
相关概念视频
01:20Fates of Pyruvate
8.7K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.7K
01:29Fermentation
115.6K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
115.6K
01:23Microbial Fermentation
84
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
84