相关实验视频

Updated: Oct 21, 2026

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Published on: July 9, 2012

一个水生环境DNA过系统,以最大限度地提高回收潜力,并促进过方法的标准化

Hayley M DeHart¹, Mark T Gasser², Jarret Dixon³

¹Research and Exploratory Development Department, The Johns Hopkins University, Laurel, MD, United States of America.

|July 17, 2023

概括

为实地和实验室使用开发了一种低成本的便携式水过系统,改进了环境DNA (eDNA) 样本采集. 这种标准化的方法增强了eDNA恢复,并促进了水生研究中的交叉研究比较.

科学领域:

环境科学环境科学
分子生物学分子生物学
生态生态学生态生态学

背景情况:

环境DNA (eDNA) 是识别水生生物的强大工具.
目前的eDNA过方法缺乏标准化,阻碍了数据的可比性.
不一致的样本处理损害了eDNA的完整性,并使研究复杂化.

研究的目的:

介绍一个简单的,低成本的,便携式的水过系统,用于水生eDNA收集.
在实地和实验室环境中提高eDNA采样标准化和效率.
为了最大限度地恢复eDNA并确保样本的完整性.

主要方法:

开发一个易于组装的,电池驱动的水过系统,耗资350美元.
使用定量PCR验证该系统对eDNA分析的有效性.
使用0.22微米和0.45微米的Sterivex过器测试过率.

主要成果:

该系统是便携式的,运行时间超过8小时,并实现高过率 (150+毫升/分钟).
收集后的即时过显著增加了实验室环境中的eDNA恢复.
在水族馆和海洋环境中都证明了成功的eDNA收集.

结论:

这种开放式硬件过系统为水生eDNA采样提供了标准化,具有成本效益的解决方案.
立即过增强了eDNA恢复和样本完整性.
预计更广泛的采用将改善eDNA研究中的交叉研究可比性.

关键词:

生物多样性生物多样性环境DNA DNA 环境DNA 现场分析现场分析侵入性物种是一种入侵性物种.我们的eDNADNA

更多相关视频

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Published on: November 25, 2016

相关概念视频

Filtration

Filtration

Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...

Microbial Wastewater Treatment

Microbial Wastewater Treatment

Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.