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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Updated: Jul 23, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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多功能开源的光记录系统多功能开源的光记录系统.

Leonhard Bandilla1

  • 1Pfahlweg 18A, 82346 Andechs, Germany.

HardwareX
|July 17, 2023
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概括
此摘要是机器生成的。

研究人员使用高功率LED和智能手机摄像头开发了一种负担得起,可适应的光检测系统. 这项创新使敏感的生物分子检测可用于各种应用,克服传统方法的成本障碍.

关键词:
光成像成像的使用方法凝电泳是一种凝电泳.宏观成像的成像方法打开硬件 打开硬件

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Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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科学领域:

  • 生物技术是生物技术.
  • 分析化学 分析化学
  • 光学传感传感器是什么?

背景情况:

  • 光检测是一种高度敏感的生物分子分析方法,通常用于凝电泳等技术.
  • 现有的光检测系统通常很昂贵,限制了可访问性和广泛采用.
  • 商业系统通常缺乏多功能性,被优化为单个应用程序和阻碍更广泛的可用性.

研究的目的:

  • 展示,描述和评估一个廉价和多功能光检测系统.
  • 通过使用可访问技术,如智能手机摄像头,使光体的敏感检测成为可能.
  • 克服当前光检测方法的成本和特定应用优化的局限性.

主要方法:

  • 使用高功率的LED发射在两个波长的样品照明.
  • 设计了一个带有角LED灯的照明设置,以确保均的照明,并最大限度地减少相机反射.
  • 包含可更换的过器框架用于波长选择,并包含LED和相机上的过器来减少背景.

主要成果:

  • 通过优化照明和过策略,实现了光体的高灵敏度检测.
  • 通过使用两个激发波长和可更换过器来证明系统的多功能性.
  • 系统设计允许明亮,均的照明,适合用智能手机摄像头进行检测.

结论:

  • 开发的系统为光检测提供了一个具有成本效益和多功能性的替代方案.
  • 它对不同光体和应用的适应性使敏感的生物分子分析更容易获得.
  • 这种方法使先进的检测技术民主化,而先前的高设备成本阻碍了这种技术的发展.