CsMYB15积极调节Cs4CL2介导的红素生物合成,在腹色的果汁囊颗粒化过程中
Fang Song1,2, Zixuan Li1, Ce Wang1
1Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees, Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, China.
Frontiers in plant science
|July 17, 2023
概括
一个关键的基因,CsMYB15,在腹子中被确定,该基因调节着素生物合成. 这一发现为防止果实颗粒化提供了洞察力,这是影响果品质的疾病.
科学领域:
- 植物分子生物学 植物分子生物学
- 农业科学 农业科学
- 生物化学 生物化学
背景情况:
- 果颗粒化是一种生理障碍,导致果汁袋的木质化和脱水,显著降低了晚熟的腹子的商业价值.
- 低冬季温度与收获前的腹子颗粒化有关,但潜在的分子机制仍然不清楚.
- 了解颗粒的遗传调节对于改善类水果的质量和市场性至关重要.
研究的目的:
- 为了识别和表征一种转录因子,它参与了晚熟的腹子的低温诱导颗粒化.
- 阐明这种转录因子调节素生物合成的分子机制及其在水果颗粒化中的作用.
主要方法:
- 鉴定和克隆SG2类型R2R3-MYB转录因子,CsMYB15,来自Citrus sinensis的克隆.
- 在低温和颗粒条件下进行亚细胞局部化,转录激活检测和基因表达分析.
- 通过腹色细胞的过度表达和瓜和腹色组织的短暂转化进行功能分析;酵母一混合体,EMSA和LUC测试以确定促进体结合和激活.
主要成果:
- 果汁囊颗粒和低温处理都显著诱导了CsMYB15的表达.
- 过度表达CsMYB15导致转基因类组织中素含量增加.
- CsMYB15直接与Cs4CL2的促进体结合并激活其表达,从而促进素的积累.
结论:
- CsMYB15在调节类植物中Cs4CL2介导的素生物合成中发挥着关键作用.
- 这项研究为晚熟的腹子中果汁囊颗粒化的转录调节机制提供了新的见解.
- CsMYB15代表了减轻类水果颗粒化的遗传策略的潜在目标.
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