相关实验视频

Updated: Jul 23, 2025

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

调节谷氨酸对皮质神经突触的影响:对HIV-1相关的神经病变发生的影响

Jianuo Liu¹, Jinyan Xie^1,2, Debashis Dutta¹

¹Neurophysiology Laboratory, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center Omaha, NE 68198-5880, USA.

International journal of physiology, pathophysiology and pharmacology

|July 17, 2023

概括

艾滋病病毒-1 艾滋病病毒-1

科学领域:

神经科学是一个神经科学.
病毒学病毒学
免疫学免疫学免疫学

背景情况:

人类免疫缺陷病毒1型 (HIV-1) 尽管结合抗逆转录病毒疗法 (cART) 在大脑中持续存在.
艾滋病毒-1 gp120蛋白与N-甲基-D-酸盐受体 (NMDARs) 相互作用,导致神经元损伤.
谷氨酸失调会影响认知功能和神经认知.

研究的目的:

为了研究HIV-1 gp120调节谷氨酸功能的假设,改变突触传输.
检查gp120和谷氨酸对神经元培养中的NMDAR介导电流和树突棘的影响.

主要方法:

在大鼠皮层神经元中研究了NMDAR介导的自发刺激后突触电流 (sEPSC_NMDAR).
分析了对gp120和谷氨酸酸的反应中的动态树突性脊柱变化.
利用CXCR4抑制剂和NMDAR对抗剂来研究机制.

主要成果:

低度的gp120和单独的谷氨酸没有显著的影响.
结合gp120和谷氨酸增加了SEPSC_NMDAR的频率,并减少了树突脊柱的数量.
这些效应被CXCR4和NMDAR对抗剂阻断.

结论:

艾滋病毒-1 gp120可能通过调节谷氨酸来改变突触功能,从而导致神经病变发生.
化基因受体CXCR4和NMDARs与gp120对谷氨酸信号传递的影响有关.
研究结果表明,在cART时代,HIV-1相关的神经认知障碍的机制存在.

关键词:

艾滋病毒-1 gp120120 的这种NMDA受体是NMDA受体.皮层神经元的皮层神经元谷氨酸酸盐的使用方法通过神经调节进行神经调节.sEPSC 这是一个很好的选择.

更多相关视频

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

Identification of EcoHIV-Infected Cells in Microglia-Manipulated Transgenic Mice

Identification of EcoHIV-Infected Cells in Microglia-Manipulated Transgenic Mice

Published on: December 20, 2024

相关概念视频

Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein

Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein

Antiepileptic drugs, such as levetiracetam (Keppra) and brivaracetam (Briviact), have emerged as crucial tools in managing epilepsy. These medications exert their therapeutic effects by targeting the synaptic vesicle protein SV2A, a transmembrane glycoprotein primarily found in the brain.
SV2A is a transmembrane glycoprotein located predominantly in the brain, modulating the release of neurotransmitters for neuronal communication. Both levetiracetam and brivaracetam exhibit a high affinity for...

Excitatory and Inhibitory Effects of Neurotransmitters

Excitatory and Inhibitory Effects of Neurotransmitters

When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of...