叶酸生物合成基因在不同来源的乳酸细菌中的发生
Fenny Amilia Mahara1, Lilis Nuraida1,2, Hanifah Nuryani Lioe1
1Department of Food Science and Technology, Faculty of Agricultural Engineering, and Technology, IPB University (Bogor Agricultural University), 16680 Bogor, Indonesia.
Food technology and biotechnology
|July 17, 2023
概括
叶酸的产生在乳酸细菌 (LAB) 中有所不同,受特定基因的影响. 这项研究在印尼LAB中确定了叶酸生物合成基因,发现这些基因的存在与叶酸生产相关,有助于选工作.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 食品科学 食品科学 食品科学
背景情况:
- 乳酸细菌 (LAB) 是已知的叶酸生产者,这是一个重要的B族维生素.
- 叶酸生产能力高度依赖菌株,并受到基因存在和调节的影响.
- 了解LAB中的叶酸生物合成对于优化其在食品应用中的生产至关重要.
研究的目的:
- 选和识别印尼食品来源的叶酸生产和非叶酸生产LAB中的叶酸生物合成基因.
- 在已识别的LAB分离物中分析细胞外叶酸的生产力.
- 了解不同LAB物种和菌株中叶酸生物合成调节的遗传基础.
主要方法:
- LAB与印度尼西亚各种当地食物来源的隔离.
- 对八个关键叶酸生物合成基因 (folE,folQ,folB,folK,folP,folC,folA) 的查和鉴定.
- 使用二极管阵列探测器 (HPLC-DAD) 的高性能液体染色学量化细胞外叶酸.
主要成果:
- 13个LAB分离物中的11个具有所有8个叶酸生物合成基因,并产生细胞外叶酸 (10.3731.10μg/mL).
- 两种非叶酸分离体缺少必要的基因 (folQ,folP,folA),这表明新叶酸合成受损.
- 遗传学分析表明,叶酸生产LAB的叶酸生物合成基因 (不包括folK和folP) 与其他LAB物种的同类基因具有单性.
结论:
- 特定的叶酸生物合成基因的存在是LAB中的叶酸生产的关键决定因素.
- 叶酸生物合成标记基因可用于叶酸生产LAB的基因型查.
- 这项研究提供了基本数据,以了解并可能增强LAB中的叶酸合成.
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