在SARS-CoV-2菌株特定的抗尖峰IgG ELISA中,使用丝虫产生的尖峰蛋白
Takeyuki Goto1, Tomoki Sasaki2,3, Yong Chong1
1Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences (The First Department of Internal Medicine), Fukuoka, Japan.
Human antibodies
|July 17, 2023
概括
使用丝虫产生的蛋白质的新ELISA有效测量SARS-CoV-2抗体水平. 这种具有成本效益的方法准确量化了感染或接种疫苗的菌株特定IgG抗体.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 病毒学 病毒学
背景情况:
- 开发具有成本效益和环保的方法来评估大量人群中对SARS-CoV-2的幽默免疫力至关重要.
- 目前用于定量SARS-CoV-2特异性抗体的方法可能昂贵且难以广泛使用.
研究的目的:
- 评估一种与酶相关的免疫吸收试验 (ELISA) 的性能,该试验使用丝虫产生的蛋白质来量化菌株特定的抗尖峰IgG (抗S IgG) 标位.
- 为了确定测试对不同SARS-CoV-2菌株,疫苗接种和感染引起的抗体反应的区分和定量能力.
主要方法:
- 开发了一种使用丝虫产生的复合蛋白的ELISA,用于野生型,三角形和奥米克朗 (BA.1) SARS-CoV-2菌株.
- 用抗His标签抗体作为标准测量光学密度 (OD) 值.
- 与艾伯特SARS-CoV-2 IgG II Quant测试对野生类型的相关ELISA结果,使用来自9名具有不同感染和疫苗接种历史的参与者的血清.
主要成果:
- 在野生型 (0.994),Delta (0.994) 和Omicron (0.996) 菌株试验中,证明了具有高确定系数 (R2) 的线性剂量反应.
- 在野生型 (0.959) 和三角型 (0.892) 菌株的ELISA和商业试验之间获得了高相关系数,对于Omicron (0.563) 有中等相关性.
- 在所有菌株中,与未接种疫苗的感染个体相比,在增剂疫苗接种者中观察到类似或更高的抗体标位. 感染Omicron的未接种疫苗的个体比接种疫苗的个体具有较低的野生型抗体标位.
结论:
- 开发的ELISA,使用丝虫产生的蛋白质,是区分和量化菌株特异性抗S IgG抗体的可行工具.
- 这种方法提供了一种具有成本效益和潜在的环保方法,用于在大型人口研究中评估针对SARS-CoV-2的幽默免疫力.
- 该测试为接种疫苗和感染不同SARS-CoV-2变种后的抗体反应提供了宝贵的见解.
相关概念视频
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:29Coronavirus
Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...