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相关概念视频

Myocarditis II: Clinical Features and Diagnostic Tests01:27

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Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...
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相关实验视频

Updated: Jul 23, 2025

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
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在囊性疾病中BRAF测试方式:比较分析和拟议的测试算法.

Aldo A Acosta-Medina1, Jithma P Abeykoon2, Ronald S Go2

  • 1Department of Internal Medicine, Mayo Clinic, Rochester, MN, US.

American journal of clinical pathology
|July 17, 2023
PubMed
概括
此摘要是机器生成的。

免疫组织化学 (IHC) 是在囊细胞性疾病中检测BRAFV600E突变的特定测试,但可以产生错误的负结果. 建议在IHC阴性时进行反射分子测试,以确保准确的诊断.

关键词:
在 BRAF 中,BRAF 基因酶临床病理学 临床病理学质细胞形成的原因是histiocytosis免疫组织化学 免疫组织化学序列分析分析的序列分析.

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An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis
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Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
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科学领域:

  • 在瘤学瘤学.
  • 病理学 病理学 病理学
  • 遗传学 遗传学 是一个

背景情况:

  • 囊性疾病越来越多地通过线粒激活蛋白激酶 (MAPK) 途径突变的透视来理解,特别是BRAFV600E.
  • 在这些疾病中评估BRAFV600E突变的最佳诊断策略仍未确定.

研究的目的:

  • 为了比较BRAFV600E突变检测的不同测试方式的性能.
  • 提出一个框架,用于评估BRAFV600E突变状态在囊细胞性疾病.

主要方法:

  • 对120名患有囊性疾病的患者进行了回顾性审查,这些患者在损伤组织上进行了BRAF突变测试.
  • 评估免疫组织化学 (IHC),聚合酶链反应 (PCR) 和下一代测序 (NGS) 的性能.

主要成果:

  • 与NGS作为黄金标准相比,IHC在BRAFV600E检测方面表现出高特异性 (96.4%),但适度灵敏度 (82.4%).
  • 虚假阴性与IHC与低变异基频率或脱组织有关;虚假阳性很少见.
  • PCR显示高一致性与NGS,在14名患者中只观察到一个不一致.

结论:

  • IHC是一种高度特定的方法来检测BRAFV600E突变,但对虚假阴性和无法检测非BRAFV600E突变有局限性.
  • 一项拟议的策略涉及使用IHC进行初始查,然后进行反射分子测试 (PCR或NGS) 以检测负结果,以确保全面的诊断准确性.