尼斯能识别小鼠心室纤维细胞的亚群,并参与细胞迁移
Vanessa Hertig1, Louis Villeneuve1, Angelino Calderone1,2
1Research Center, Montreal Heart Institute, Montréal, Québec, Canada.
American journal of physiology. Cell physiology
|July 17, 2023
概括
纤维细胞原生细胞表达内斯,这是细胞迁移至关重要的蛋白质. 尼斯促进腹腔纤维细胞迁移,是前体细胞的标记物,影响心脏修复和重塑.
科学领域:
- 心血管生物学 心血管生物学
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 纤维细胞原生细胞迁移对于心脏发育和受伤后的修复至关重要.
- 尼斯是一种中间丝蛋白,与细胞迁移有关,在痕肌纤维细胞中发现.
- 尼斯在纤维细胞原生细胞迁移和表型中的作用仍然不清楚.
研究的目的:
- 为了调查纤维细胞原生细胞是否表达内斯.
- 为了确定雀巢是否驱动心室纤维细胞的迁徙表型.
- 探索内斯在心脏重塑中的作用.
主要方法:
- 在胚胎Tcf21/WT1-纤维细胞原生细胞中确定了nestin表达.
- 在新生儿和成年大鼠心室纤维细胞中分析了nestin和Tcf21/WT1表达.
- 用于在体外迁移试验的新生小鼠腹腔纤维细胞中消耗尼斯的lentiviral shRNA.
- 在Matrigel中检查了痕衍生的肌纤维细胞迁移和光样结构的形成.
主要成果:
- 胚胎Tcf21/WT1-纤维细胞原生细胞主要表达于内斯丁.
- 新生小鼠腹腔纤维细胞显示出共表达Tcf21/WT1和nestin的亚种群,该亚种群对细胞循环重新进入具有耐火性.
- 来自痕的肌纤维细胞形成了类似光的结构,具有内阳性膜投射.
- 新生小鼠腹腔纤维细胞中的内斯耗尽显著抑制了它们的迁移反应.
结论:
- 尼斯是胚胎Tcf21/WT1阳性纤维细胞原生细胞的标记物.
- 新生小鼠腹腔纤维细胞的一个明显的亚群表达了nestin和Tcf21/WT1,对细胞循环刺激表现出抵抗力.
- 在心脏重塑过程中,内斯可能促进腹腔纤维细胞迁移.
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