接触模式原子力显微镜作为一种用于形态观测和细菌细胞损伤分析的快速技术
Héctor Pérez Ladrón de Guevara1, Virginia Villa-Cruz2, Rita Patakfalvi1
1Centro Universitario de los Lagos, Universidad de Guadalajara.
Journal of visualized experiments : JoVE
|July 17, 2023
概括
原子力显微镜 (AFM) 为细菌形态和细胞损伤特征提供高分辨率的3D成像. 这种廉价的技术有效地可视化了纳米粒子对细菌的影响,性能优于电子显微镜.
科学领域:
- 显微镜的使用方法
- 纳米技术纳米技术
- 细菌学 细菌学是一门学科.
背景情况:
- 电子显微镜是细胞结构表征的标准工具,但复杂且昂贵.
- 原子力显微镜 (AFM) 提供高分辨率的3D成像,没有真空或导电性要求,适用于各种样品.
- AFM提供安格斯特罗姆到微米尺度的地形信息,使用探头扫描表面.
研究的目的:
- 为细菌形态学和细胞损伤评估提出和验证原子力显微镜 (AFM).
- 使用AFM研究纳米粒子 (NP) 对细菌细胞结构的影响.
- 将AFM的实用性与用于细菌分析的传统电子显微镜进行比较.
主要方法:
- 细菌菌株 (金黄色葡萄球菌,大肠杆菌,Pseudomonas hunanensis) 进行了培养.
- 黄金葡萄球菌和大肠杆菌在不同度的纳米颗粒 (NP) 中进行了化.
- 细菌悬浮被固定在玻璃支架上,并使用多个尺度的接触AFM获得图像.
主要成果:
- AFM成功地可视化了细菌菌株的独特形态特征.
- 该研究观察并描述了因纳米粒子暴露引起的细菌细胞损伤.
- AFM图像提供了NP诱导的细菌细胞结构变化的明确证据.
结论:
- 接触式AFM是一种可行且具有成本效益的方法,用于表征固定细菌细胞的形态.
- AFM是研究纳米颗粒对细菌完整性的影响的合适工具.
- 在细菌研究中,AFM为电子显微镜提供了一个可访问和经济的替代方案.
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