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在废水和空气样本中的SARS-CoV-2RNA的量化和全基因组表征

José Gonçalves1, Priscilla Gomes da Silva2, Tom Koritnik3

  • 1Institute of Sustainable Processes, University of Valladolid; Department of Chemical Engineering and Environmental Technology, University of Valladolid; Department of Public Health Microbiology, National Laboratory of Health, Environment and Food; zemcg5@gmail.com.

Journal of visualized experiments : JoVE
|July 17, 2023
PubMed
概括

基于废水的流行病学为跟踪SARS-CoV-2提供了一种强大的方法. 这项研究适应了这些技术来检测和量化包括SARS-CoV-2在内的病毒,在废水和空气样本中.

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Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...