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工程化肠道共生细菌媒介RNAi用于有效控制幼虫

Jinjin Ding^1,2,3,4, Chunlai Cui^2,3,4, Guandong Wang^2,3,4

¹School of Life Science, East China Normal University, Shanghai, China.

Microbiology spectrum

|July 17, 2023

概括

工程化肠道细菌提供RNA干扰 (RNAi) 来控制携带疟疾的蚊子幼虫. 这种新的共生介导RNAi (smRNAi) 方法抑制了关键基因,抑制了Anopheles stephensi的发育和增加死亡率.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

阿诺菲莱斯蚊子传播疟疾,这是全球主要的健康威胁.
在非洲的Anopheles stephensi入侵使疟疾根除复杂化,原因是抗杀虫剂和户外咬伤.
RNA干扰 (RNAi) 通过沉默目标基因,为害虫控制提供了一个潜在的策略.

研究的目的:

开发一种基于RNAi的新策略,用于控制Anopheles stephensi幼虫.
为了设计一个肠道共生细菌,以有效地传递dRNA和在蚊子幼虫中的基因沉默.
评估共生介导RNAi (smRNAi) 在抑制蚊子发育和生存方面的疗效.

主要方法:

通过删除其RNase III基因来产生dsRNAs的肠道细菌Serratia fonticola进行了工程设计.
使用CRISPR-Cas9进行S. fonticola.基因编辑.
向Anopheles stephensi幼虫注射工程S. fonticola,以诱导向Met和EcR基因的RNAi.

主要成果:

工程设计的S. fonticola菌株稳定地殖民了蚊子幼虫的肠道.
生产的dsRNAs (dsMet, dsEcR) 通过RNAi有效抑制了目标基因表达.
显著抑制了Anopheles stephensi幼虫的发育,导致高死亡率.

结论:

交生体介导的RNAi (smRNAi) 是一种可行且可持续的方法来控制蚊子幼虫.
工程化肠道细菌为昆虫基因沉默提供了有效的dsRNA传递系统.
这种方法提供了一种有希望的策略,通过准载体种群来打击疟疾.

关键词:

RNase III 第三部分 dsRNARNA是什么意思肠道共生细菌肠道共生细菌蚊子蚊子蚊子同生体介导的RNAi.

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