用SS-31针对老年大脑血管中的线粒体,这是对大脑微血管的蛋白质学研究
Abigail Seman1, Partha K Chandra1,2, Stephanie D Byrum3
1Department of Pharmacology, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA.
GeroScience
|July 17, 2023
概括
线粒体功能障碍有助于大脑衰老. 药物SS-31针对线粒体,改变了大脑的微血管蛋白质,这表明线粒体是老化大脑的治疗目标.
科学领域:
- 神经科学是一个神经科学.
- 老年学是一门学科.
- 生物化学 生化学
背景情况:
- 老化的大脑经历了与认知衰退和阿尔茨海默氏症等疾病相关的微血管变化.
- 线粒体功能障碍是与年龄相关的微血管变化的关键因素.
- 在衰老期间大脑微血管系统中向线粒体的药物的治疗潜力仍然未被探索.
研究的目的:
- 调查SS-31针对线粒体是否可以减轻大脑微血管蛋白质组中与衰老相关的变化.
- 评估SS-31对大脑微血管结构中的线粒体和非线粒体蛋白质的影响.
主要方法:
- 老年雄性小鼠被治疗为线粒体向的四分SS-31或载体控制.
- 使用激光光斑成像测量脑血流 (CBF).
- 用Orbitrap Eclipse Tribrid质谱法对孤立的皮质微容器进行了蛋白质组分析.
主要成果:
- 虽然CBF保持不变,但蛋白质组分析显示SS-31和载体组之间的皮质微血管蛋白质丰度有显著差异.
- 在6267种已识别的蛋白质中,12%与线粒体相关,其中107种具有显著差异表达.
- 不同表达的蛋白质与氧化酸化,新陈代谢,抗氧化防御和线粒体动力学有关,SS-31也影响非线粒体蛋白质.
结论:
- 大脑微血管结构中的线粒体代表了对抗大脑衰老的可行治疗标.
- 在老化过程中,SS-31的再生效应可能源于大脑微血管结构中广泛的蛋白质组变化.
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