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在脂质膜中包装异型微凝颗粒:颗粒形状和膜刚性的影响

Xiaoyan Liu^1,2, Thorsten Auth³, Nabanita Hazra⁴

¹School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.

Proceedings of the National Academy of Sciences of the United States of America

|July 17, 2023

概括

粒子形状和膜特性控制细胞吸收. 圆形粒子促进了比球形粒子更深的膜包裹,挑战了理论预测,并表明粘附强度随着粒子拉伸而增加.

科学领域:

生物物理学的生物物理.
材料科学材料科学材料科学
纳米技术纳米技术

背景情况:

细胞通过内分细胞分裂吸收纳米颗粒对于药物输送和理解纳米毒性至关重要.
粒子与膜的相互作用决定了粒子是否吸附,包裹或穿过细胞膜.
控制这些相互作用是设计有效纳米药物和评估环境风险的关键.

研究的目的:

为了研究粒子形状,粘附能量,膜相态度和曲刚性如何影响脂质膜包裹的体颗粒.
探索粒子几何和膜包裹的能量之间的关系.
为生物材料和治疗应用提供纳米粒子膜相互作用设计的见解.

主要方法:

利用软核微凝颗粒 (球形和圆形) 和具有不同组成的脂膜的模型系统.
使用共聚焦显微镜可视化和分析粒子包裹,膜变形和膜上的粒子组织.
进行理论计算,将实验结果与基于固定的粘合强度的预测进行比较.

主要成果:

证明调整粒子和膜性质可以指导包裹,膜变形和粒子组织.
观察到,与体积相似的球形微凝颗粒相比,圆形微凝颗粒的深包裹状态更有利.
实验结果与固定粘附的理论预测相矛盾,这意味着粘附强度随着粒子拉伸而增加.

结论:

粒子形状显著影响膜包装,圆形粒子显示增强的包装.
实验和理论结果之间的差异凸显了粒子膜粘附的动态性质,随着粒子变形而增加.
研究结果为设计新生物材料和优化基于纳米粒子的治疗策略提供了宝贵的指导.

关键词:

异型的微凝是一种异型的微凝.巨大的单状囊泡.脂质膜是一种脂质膜.膜粘附膜粘附是一种包装包装包装

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