DbyDeep:通过深度学习探索MS可检测
Juho Son1, Seungjin Na1,2, Eunok Paek1,2
1Department of Computer Science, Hanyang University, Seoul 04763, Republic of Korea.
Analytical chemistry
|July 17, 2023
概括
在质谱法 (MS) 中检测对蛋白质组学至关重要. 一个新的深度学习模型,DbyDeep,通过考虑不仅仅是序列,提高了的检测性预测.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 计算生物学 计算生物学
- 质谱测量质量谱测量
背景情况:
- 对于基于质谱 (MS) 的蛋白质组学,特别是针对性蛋白质组学,的检测性预测至关重要.
- 当前的机器学习方法通常仅依赖于序列或物理化学性质.
- 的检测受到测序之外的实验因素的影响,包括样品准备,消化,分离,电离和MS参数.
研究的目的:
- 开发一种改进的计算模型,用于预测质谱学中的检测能力.
- 将超越序的上下文信息纳入预测模型.
- 与现有方法相比,评估新模型的性能.
主要方法:
- 开发了DbyDeep,这是一个端到端的长短期存储器 (LSTM) 网络.
- 将序列上下文和蛋白酶分裂部位上下文纳入深度学习模型.
- 在多个MS/MS数据集上评估了DbyDeep,跨越多种物种和MS仪器.
主要成果:
- 与现有方法相比,DbyDeep在预测的检测性方面表现优异.
- 包括分离点上下文在内显著提高了预测准确度.
- 该模型成功地预测了来自不同数据集的,表明了强度.
结论:
- 为了准确预测的可检测性,需要一个包含多个上下文因素的全面学习模型.
- DbyDeep 通过考虑序列和裂痕部位的背景,提供了更有效的方法.
- 这些发现强调了实验背景在基于MS的蛋白质组学分析中的重要性.
更多相关视频
09:32Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
12.4K
09:47Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches
Published on: December 15, 2023
1.1K
相关概念视频
01:33Peptide Identification Using Tandem Mass Spectrometry
6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
01:19MALDI-TOF Mass Spectrometry
5.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.0K