在自然月经周期中,在体外受精和胚胎移植之前,早期分泌和中期分泌子宫内膜的转录组图案
Sung Pil Choo1, Inha Lee2,3, Jae-Hoon Lee2,3
1Department of Obstetrics and Gynecology, Inha University Hospital, College of Medicine, Inha University, Incheon, Korea.
Obstetrics & gynecology science
|July 17, 2023
概括
在IVF-ET之前,子宫内膜基因表达在早期和中期分泌阶段之间存在差异,随着怀孕结果而变化. 这些基因模式可能表明子宫内膜对植入的接受性.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 在体外受精过程中受精.
背景情况:
- 在体外受精和胚胎移植 (IVF-ET) 期间,子宫内膜的受容性对于成功植入至关重要.
- 了解月经周期内子宫内膜的转录组变化是提高试管婴儿-ET成功率的关键.
研究的目的:
- 在IVF-ET之前,调查早期分泌阶段 (ESP) 和中期分泌阶段 (MSP) 之间的子宫内膜基因表达模式的差异.
- 在接受IVF-ET的患者中,确定与怀孕结果相关的差异表达基因 (DEG).
主要方法:
- 在IVF-ET之前,从ESP或MSP的30名患者收集了子宫内膜组织样本.
- 组织学约会证实ESP (16-18天) 和MSP (19-21天) 使用诺耶斯标准.
- 使用NanoString nCounter技术在MSP中识别DEG,与孕妇和非孕妇组中的ESP相比.
主要成果:
- 在比较MSP与ESP时,在孕妇组中发现了13个DEG,在非孕妇组中发现了11个DEG.
- 包括ADAMTS15和TMEM252在内的几种基因在MSP和ESP中都被上调调.
- 特定的基因,如TMEM37和细胞染色体P450家族24亚家族A成员1在MSP上调仅在孕妇组.
结论:
- 在IVF-ET之前的子宫内膜转录组图案根据IVF-ET结果不同.
- 发现的新型DEG可能在植入窗口中发挥作用.
- 这些DEG可以作为评估子宫内膜受体性的潜在生物标志物.
相关概念视频
Secretory Phase
865
The secretory phase of the menstrual cycle, spanning from day 14 to 28 in a typical 28-day cycle, is a period of significant physiological changes in the female reproductive system. This phase commences immediately after ovulation and is characterized by the preparation of the endometrium for potential embryo implantation.
Following ovulation, the corpus luteum, a temporary endocrine structure, produces progesterone and estrogens. These hormones stimulate the growth and coiling of endometrial...
Following ovulation, the corpus luteum, a temporary endocrine structure, produces progesterone and estrogens. These hormones stimulate the growth and coiling of endometrial...
865
Ovarian Cycle
1.4K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
1.4K
Hormonal Regulation of the Menstrual Cycle
456
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
456
The Menstrual Cycle
926
The menstrual cycle is a recurrent sequence of changes in the uterine endometrium, specifically its functional layer, the stratum functionalis. This cycle prepares the uterus for potential pregnancy. This cycle typically spans 21–35 days, averaging 28 days, and aligns with the ovarian cycle, regulated by fluctuating levels of ovarian hormones, primarily estrogen and progesterone.
The menstrual phase occurs from days 1 to 5 and involves the shedding of the stratum functionalis, as a...
The menstrual phase occurs from days 1 to 5 and involves the shedding of the stratum functionalis, as a...
926
Proliferative Phase
552
The proliferative phase typically occurs after menstruation and lasts between 6 to 13 days in a standard 28-day cycle. This phase involves the reconstruction of the endometrium, guided by estrogen produced by the developing ovarian follicle.
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
552


