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在基因毒性应激反应中分离相位的核糖体新生链复合体

Orsolya Németh-Szatmári¹, Bence Nagy-Mikó¹, Ádám Györkei^2,3

¹Department of Biochemistry and Molecular Biology, University of Szeged, 6726 Szeged, Hungary.

RNA (New York, N.Y.)

|July 17, 2023

概括

组合体,核蛋白复合体,参与DNA修复. 这些在酵母和人体细胞中发现的结构,动态地对DNA损伤作出反应,这表明它们在细胞修复机制中的作用.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

组合体是EDTA和RNase耐药的核糖蛋白 (RNP) 复合体,由暂停的核糖体和新生的多链组成.
之前的研究在酵母和人类细胞中确定了与蛋白质酶子单元Rpt1相关的组合体,突出显示了无序的N终端新生链的作用.

研究的目的:

通过生物信息学和实验验证,识别一些相关RNP的新组合.
调查组合体在DNA修复途径中的作用及其对DNA损伤刺激的反应.

主要方法:

酵母蛋白复合体的生物信息分析,以预测N终端疾病的倾向.
使用酵母Sgs1 DNA酶和人类BLM酶进行实验验证.
组基因组相关RNAs的RNA测序.
传输电子显微镜用于可视化细胞结构.

主要成果:

生物信息学预测了DNA修复蛋白在潜在的组合体组件中进行丰富.
新生的Sgs1链形成组合体,SGS1mRNA在紫外线暴露时动态转移.
人类BLM螺旋酶在组合体中被确定,并为DNA修复蛋白的mRNA进行了丰富.
1,6-二醇破坏了组合类结构的组合,并增加了对X射线治疗的敏感性.

结论:

集合体与酵母和人体细胞中的DNA修复蛋白有关.
组合的动力学受到外部刺激的调节,特别是DNA损伤.
这些发现表明,聚合体在物种间的DNA损伤反应中起着重要作用.

关键词:

这里是BLM.对DNA损伤的反应反应在Sgs1中,Sgs1是Sgs1.大会总结了一些总结.阶段分离的分离阶段分离.

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