相关实验视频
Updated: Jul 23, 2025

15:53
Isolation and Biophysical Study of Fruit Cuticles
Published on: March 30, 2012
16.8K
COPII的CsSEC23子单元调解了黄瓜中的水果光泽度
Luyao Gao1,2,3, Jiajian Cao1,2,3,4, Siyu Gong5
1College of Horticulture, Hunan Agricultural University, Changsha, 410128, China.
The Plant journal : for cell and molecular biology
|July 17, 2023
概括
一种新的黄瓜突变发现,CsSEC23是COPII囊泡的组成部分,调节水果皮的光泽. 这种突变影响和皮质的运输,影响皮质结构和光泽.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 黄瓜的光泽度是一个重要的水果质量特征.
- 调节黄瓜皮光泽的分子机制尚未完全理解.
研究的目的:
- 为了确定黄瓜光泽的遗传基础.
- 阐明COPII囊泡运输在黄瓜果皮发育中的作用.
主要方法:
- 使用MutMap,基因型和基因编辑来识别致病基因.
- 分析蛋白质相互作用,基因表达和皮质结构.
主要成果:
- 一种新的黄瓜光泽皮 (Csgp) 突变被确定,由CsSEC23.3中的突变引起.
- 一个核心的COPII囊泡组件CsSEC23对于黄瓜的光泽至关重要.
- 突变破坏了CsSEC23-CsSEC31的相互作用,损害了COPII囊泡的运输.
- 参与和皮质运输的基因被上调,突变体的皮质结构被改变.
结论:
- CsSEC23在调节黄瓜果皮的光泽方面发挥着至关重要的作用.
- 在光泽度调节中的CsSEC23的功能与COPII囊泡介导的和切片的运输有关.
相关概念视频
Coat Assembly and GTPases
3.6K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.6K
Fruit Development, Structure, and Function
22.5K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
22.5K
COP Coated Vesicles
7.9K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
7.9K
Seed Structure and Early Development of the Sporophyte
28.5K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
28.5K
Pinching-off of Coated Vesicles
3.2K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.2K
Role of Microtubules in Cell Wall Deposition
2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K

