对酸盐代谢变化的适应性反应影响了牛胚胎的表观遗传景观和转录组学
Aldcejam Martins da Fonseca Junior1, Jessica Ispada1, Erika Cristina Dos Santos1
1Federal University of ABC - Center for Natural and Human Sciences, Av. Dos Estados, 5001, Bairro Santa Terezinha, Bloco A, Lab 504-3, Santo André, SP, CEP: 09210-580, Brazil.
Scientific reports
|July 17, 2023
概括
胚胎培养条件影响表观遗传重编程. 用DCA或IA操纵pyruvate代谢改变了牛胚胎发育,细胞数和基因表达,突出显示了代谢和表观遗传学之间的联系.
科学领域:
- 胚胎学 胚胎学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 代谢调节 代谢调节 代谢调节 代谢调节
背景情况:
- 表观遗传重编程对于早期胚胎发育至关重要.
- 胚胎代谢状态越来越被认为是表观遗传事件的关键调节者.
研究的目的:
- 调查牛胚胎中酸盐代谢和表观遗传调节之间的关系.
- 评估二乙酸盐 (DCA) 和乙酸盐 (IA) 对早期胚胎发育和表观遗传修饰的影响.
主要方法:
- 牛胚胎从第5天开始用DCA (pyruvate类似物) 或IA (GAPDH抑制剂) 进行培养.
- 分析了线粒体膜潜力,乙-CoA,ATP-酸酶,以及组织蛋白修饰 (H3K9ac,H3K27ac,H3K27me3).
- 进行了转录组分析和细胞计数.
主要成果:
- 最初,DCA和IA都增加了线粒体膜的潜力,但胚芽细胞的水平较低,这表明代谢适应.
- 代谢变化导致全球H3K9和H3K27乙化和H3K27三甲基化模式的变化.
- 转录组分析显示了代谢过程,表观遗传调节,基因表达控制和细胞循环途径的显著差异.
- 与对照组相比,治疗组的总细胞数和细胞分化都发生了变化.
结论:
- 酸盐的新陈代谢显著影响了在植入前牛胚胎中的表观遗传重编程.
- 培养系统对于精确的表观遗传重编程至关重要,影响分子控制和细胞分化.
- 这些发现加强了能量代谢与早期发育过程中的表观遗传调节之间的复杂联系.
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