PpMYB105通过调节桃子果中的PpMsrA1来抑制冷却损伤
Caifeng Jiao1, Yancheng Wei2, Jing Dong2
1School of Horticulture, Anhui Agricultural University, Hefei, 230036, People's Republic of China. anyeweiyangjiao@126.com.
Plant cell reports
|July 17, 2023
概括
甲基斯酸盐 (MeJA) 通过上调PpMYB105来增强桃子的冷却耐受性,从而促进甲二硫酸减少酶A1 (PpMsrA1) 基因表达,减轻冷藏损伤.
科学领域:
- 植物生理学 植物生理学
- 收获后生物学 收获后生物学
- 分子生物学分子生物学
背景情况:
- 收获后的水果质量通过冷藏来保持,但桃子容易受到冷却损伤 (CI).
- 桃子的冷却损伤导致了重大的经济损失.
- 制定减轻CI的策略对于水果行业至关重要.
研究的目的:
- 调查甲基斯酸盐 (MeJA) 在提高桃子冷却耐受性的作用.
- 阐明MeJA改善冷藏品质量的分子机制.
- 为了确定关键的基因和参与MeJA介导冷却耐受性的调节途径.
主要方法:
- 将MeJA应用于桃子,并评估CI的严重程度.
- 对甲氨酸硫氧化物减少酶A1 (PpMsrA1) 和PpMYB105.5的基因表达分析.
- 酶活性测定MsrA和测量氨酸 (Met) 和氨酸-S-硫氧化物 (Met-S-SO) 含量.
- 对反应性氧物种 (ROS) 生产的分析.
- 对PpMYB105和PpMsrA1.1.进行促进体分析和转录因子结合试验.
- 在桃子和番茄中进行基因沉默和过度表达研究.
主要成果:
- 补充MeJA可以降低CI的严重程度,并增强PpMsrA1基因表达.
- MeJA的应用增加了MsrA活性,Met含量,并降低了Met-S-SO含量和ROS产量.
- PpMYB105蛋白通过结合其MYB结合元件来积极调节PpMsrA1促进体.
- 在MeJA上调调节了PpMYB105基因表达.
- 过度表达PpMYB105增强了番茄的冷却耐受性和SlmsrA1表达.
- 病毒诱导的PpMYB105基因沉默增加了CI严重程度,并降低了桃子中的PpMsrA1表达.
结论:
- 通过调节PpMYB105表达,MeJA提高了桃子的冷却耐受性.
- PpMYB105作为PpMsrA1的积极调节剂,调解MeJA诱导的冷却耐受性.
- MeJA-PpMYB105-PpMsrA1通路对于减轻收获后桃子冷却损伤至关重要.
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