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布拉西卡 (Brassica juncea) 叶皮含有和曼诺 (xylomannan),它们抑制了叶面上冰的再结晶

Kailash Yadav¹, Meenakshi Arya², Satya Prakash³

¹Molecular Plant Physiology and Proteomics Laboratory, Department of Botany, University of Delhi, Delhi, 110007, India.

|July 17, 2023

概括

布拉西卡叶中的结合糖通过抑制冰的再结晶而不是热歇斯底里表现出抗活性. 这一发现揭示了叶子皮质碳水化合物在防保护中的新作用.

科学领域:

植物生物学植物生物学
生物化学生物化学
低温生物学低温生物学

背景情况:

抗生物分子,特别是抗蛋白 (AFP),通过控制冰晶的生长来保护植物免受伤害.
虽然在植物的质体中已知AFPs,但叶片皮质的抗性质仍未被探索.

研究的目的:

为了研究Brassica juncea (BJ) 叶皮的潜在抗活性.
为了识别BJ叶子皮质中负责抗活性的生物分子.

主要方法:

皮层蛋白质丰富分量的纳米LC-MS/MS分析.
冰晶形态 (ICM) 和冰再结晶抑制 (IRI) 的测试.
分离的皮质膜和部分的酶 (xylanase,cellulase) 和化学 (FTIR,UV,NMR,TLC) 分析.

主要成果:

一种皮质提取物表现出耐热和耐酶的抗活性,具有显著的IRI (46%),但最小的热歇斯底里 (0.17°C).
FTIR,UV,NMR和酶水解表明结合糖的存在和贡献,特别是和,对抗活性.
用西兰酶和蜂酶治疗导致50%的抗活性丧失,证实了碳水化合物的作用.

结论:

布拉西卡叶皮质具有抗活性,主要由结合糖通过抑制冰的再结晶介导.
这项研究确定了叶子皮层碳水化合物在植物耐受性中的新角色,为研究抗机制开辟了新的途径.

关键词:

抗蛋白质是一种抗蛋白质.美国的FTIR是FTIR.糖是一种糖.这是热歇斯底里.

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