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能量压力诱导的circZFR通过调节替代拼接来增强肺腺癌中的氧化酸化

Zhifei Ma^1,2, Hao Chen^1,3, Zhijun Xia^1,4

¹Department of Thoracic Surgery, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing Medical University Affiliated Cancer Hospital & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing, 21009, P.R. China.

Journal of experimental & clinical cancer research : CR

|July 17, 2023

概括

循环RNA ZFR (circZFR) 通过增强氧化酸化来促进在能量压力下肺腺癌的生存. 在瘤中升高的循环ZFR与预后不佳相关,提供了潜在的治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
代谢过程中的代谢.

背景情况:

循环RNAs (circRNAs) 与癌症有关.
肺腺癌 (LUAD) 在能量压力下代谢重编程中circRNAs的作用尚不清楚.

研究的目的:

为了研究 circRNAs 在 LUAD 代谢适应能量压力的功能和机制.
为了确定LUAD的潜在治疗点.

主要方法:

循环RNA分析和葡萄糖剥夺测试以确定能量压力诱导的循环RNA.
用RNA-seq,RTCA和OCR测量来评估LUAD中的circZFR功能.
涉及circRNA pull-down,RNA免疫沉和生物信息学的机制研究.
在LUAD组织和患者衍生的异种移植中进行临床验证.

主要成果:

循环ZFR在LUAD上调并由葡萄糖剥夺诱导,增强氧化酸化 (OXPHOS) 以适应能量压力.
CircZFR表达与较高的T阶段和LUAD患者的更差预后有关.
CircZFR稳定了HNRNPLL,调节了替代拼接,并激活了AKT-mTOR通路以促进OXPHOS.

结论:

CircZFR在LUAD对能量压力的代谢适应中发挥着关键作用.
在LUAD中,CircZFR代表了抗癌代谢疗法的潜在新目标.
这项研究增强了对癌症代谢中的circRNAs和替代拼接的理解.

关键词:

另类拼接. 替代拼接.能量压力能量压力肺部腺癌瘤是肺部腺癌.氧化酸化是一种氧化酸化.环RNA 环RNA 是一个环RNA.

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