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全基因组测序和功能注释致病性Paraconiothyrium brasiliense导致人类细胞炎的功能注释

Haibing Liu¹, Yue Zhang¹, Jianguo Chen²

¹Department of Clinical Laboratory, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.

|July 17, 2023

概括

以前已知的植物疾病的真菌Paraconiothyrium brasiliense已被确定为人类眼细胞炎的原因. 基因组分析揭示了许多毒性因素,为人类的病原性提供了洞察力.

科学领域:

医学真菌学医学真菌学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

从患者的分泌物中分离出一种导致眼细胞炎的丝状真菌.
遗传学分析发现该分离物是巴西的Paraconiothyrium.
这标志着首次报告了由P. brasiliense引起的人类感染,这是一种主要以植物致病性而闻名的属.

研究的目的:

从遗传角度全面分析P. brasiliense菌株的病原性和蛋白质组学.
建立P. brasiliense.的基因组资源.
为了解人类感染机制和开发治疗方法提供理论基础.

主要方法:

使用Illumina NovaSeq和牛津纳米孔技术进行全基因组测序.
生物信息学分析包括BLAST与公共基因组数据库对比.
使用rDNA内部转录间隔区域 (ITS) 和单拷贝基因家族的家族遗传学分析.

主要成果:

巴西的GGX 413基因组为39.49 Mb,GC含量为51.2%,编码了13057个蛋白质编码基因和181个非编码RNA.
功能注释确定了592个毒性因子基因,包括61个致命的和30个高毒性因子.
关键的毒性相关基因包括碳水化合物活性酶 (54),酶 (119) 和二次代谢物合成 (27) 的基因.

结论:

这项研究提供了Paraconiothyrium brasiliense的第一个基因组数据.
已确定的毒性因素为研究其在人类感染中的致病机制提供了基础.
这些基因组资源将有助于开发未来的治疗方法和研究P. brasiliense相关疾病.

关键词:

功能注释功能注释人类病原性真菌人类病原性真菌帕拉科尼奥西 (Paraconiothyrium brasiliense) 是一种葡萄牙葡萄酒.整个基因组测序的测序

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相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.